Antibodies Reactive to Ehrlichia spp. Are Common in Oklahoma Horses
Antibodies Reactive to Ehrlichia spp. Are Common in Oklahoma Horses
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DOI：
10.1089/vbz.2013.1570
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发表时间：
2014-08-01
影响因子：
2.1
通讯作者：
Little, Susan E.
中科院分区：
文献类型：
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作者：
Carmichael, Robert C.;Duell, Jason R.;Little, Susan E.
蜱虫侵染和蜱传病原体感染在北美的马中是常见的，但马感染真正的埃立克体属。尚未描述。为了确定美国中南部马自然暴露于蜱传疾病病原体并感染蜱传疾病病原体的程度，随机（n = 240）或从有活动性蜱感染的马（n = 73）中采集血清样本，并通过免疫荧光抗体测定（IFA）和/或酶联免疫吸附测定（ELISA）检测是否存在对埃立克体属反应的抗体，无形体属，和伯氏疏螺旋体。阳性标本进一步用种特异性血清学方法检测E.犬埃立克体和chaffeensis和全血样品通过PCR检测E. canis、犬棘球绦虫E. chaffeensis、E. ewingii和E.一种类似反刍动物的微生物，称为帕诺拉山埃里希体。对埃立克体属有反应性的抗体在8.75%（21/240）的随机采集的样品和24.7%（18/73）的蜱虫感染马的血清样品中鉴定出埃立克体，但种特异性ELISA和PCR未能证实暴露于或感染任何已知的埃立克体属。无形体属的抗体（5/313; 1.6%）和B.伯氏症少见（3/313; 1.0%）。这些数据表明，在美国中南部的马很可能暴露于一种新的埃里希体属。需要进一步的研究，以确定病原体的血清学活动，并确定临床意义，如果有的话，这一发现。
Tick infestations and infection with tick-borne agents are commonly recognized in horses in North America, but equine infection with true Ehrlichia spp. has not been described. To determine the degree to which horses in the south-central United States are naturally exposed to and infected with tick-borne disease agents, serum samples were collected at random (n = 240) or from horses with active tick infestations (n = 73) and tested by immunofluorescence antibody assay (IFA) and/or enzyme-linked immunosorbent assay (ELISA) for evidence of antibodies reactive to Ehrlichia spp., Anaplasma spp., and Borrelia burgdorferi. Positive samples were further evaluated by species-specific serology for antibodies reactive to E. canis and E. chaffeensis, and whole blood samples were tested by PCR for evidence of infection with E. canis, E. chaffeensis, E. ewingii, and an E. ruminantium-like organism referred to as the Panola Mountain Ehrlichia. Antibodies reactive to Ehrlichia spp. were identified in 8.75% (21/240) of the randomly acquired samples and 24.7% (18/73) of the serum samples from tick-infested horses, but species-specific ELISA and PCR failed to confirm exposure to or infection with any known Ehrlichia spp. Antibodies to Anaplasma spp. (5/313; 1.6%) and B. burgdorferi (3/313; 1.0%) were uncommon. These data suggest that horses in the south-central United States are likely exposed to a novel Ehrlichia sp. Further research is needed to identify the etiologic agent responsible for the serologic activity seen and to determine the clinical significance, if any, of this finding.