Metabolism of apoprotein B in cynomolgus monkey: evidence for independent production of low-density lipoprotein apoprotein B.
Metabolism of apoprotein B in cynomolgus monkey: evidence for independent production of low-density lipoprotein apoprotein B.
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食蟹猴中脱辅基蛋白 B 的代谢：低密度脂蛋白脱辅基蛋白 B 独立产生的证据。
DOI：
10.1152/ajpendo.1983.244.2.e196
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Brown,WV
中科院分区：
文献类型：
--
作者：
Goldberg,IJ;Le,NA;Ginsberg,HN;PaternitiJr,JR;Brown,WV
在5只食蟹猴中研究了注射放射性碘标记的同源极低密度脂蛋白后极低密度脂蛋白载脂蛋白B的催化及其向低密度脂蛋白的转化。极低密度脂蛋白载脂蛋白B的平均（+/- SD）部分分解代谢率为0.97 +/- 0.20 h-1，平均（+/- SD）产生率为0.76 +/- 0.20 mg X kg-1 X h-1。极低密度脂蛋白载脂蛋白B转化为低密度脂蛋白的百分比范围为33 - 59%。在另外5只动物中使用同源放射性标记低密度脂蛋白进行的低密度脂蛋白载脂蛋白B转换的单独研究中，低密度脂蛋白载脂蛋白B的平均（+/- SD）部分分解代谢率为0.050 +/- 0.017 h-1，平均（+/- SD）载脂蛋白B产生率为0.70 +/- 0.18 mg X kg-1 X h-1。总低密度脂蛋白载脂蛋白B的生产与来自极低密度脂蛋白载脂蛋白B的比较表明，大部分的血浆低密度脂蛋白载脂蛋白B来自循环极低密度脂蛋白载脂蛋白B以外的来源。通过同时注射放射性标记的极低密度和低密度脂蛋白，在两只动物中证实了这一点。因此，相当大比例的食蟹猴低密度脂蛋白是通过直接肝脏分泌或低密度脂蛋白在外周循环中出现之前的快速转化产生的。
The catabolism of very-low-density lipoprotein apoprotein B and its conversion to low-density lipoprotein was studied in five chow-fed cynomolgus monkeys following injection of radioiodinated homologous very-low-density lipoproteins. The mean (+/- SD) fractional catabolic rate of very-low-density lipoprotein apoprotein B was 0.97 +/- 0.20 h-1 and the mean (+/- SD) production rate was 0.76 +/- 0.20 mg X kg-1 X h-1. The percent of conversion of very-low-density lipoprotein apoprotein B to low-density lipoprotein ranged from 33 to 59%. In separate studies of low-density lipoprotein apoprotein B turnover performed using homologous radiolabeled low-density lipoprotein in five additional animals, the mean (+/- SD) fractional catabolic rate for low-density lipoprotein apoprotein B was 0.050 +/- 0.017 h-1 and the mean (+/- SD) apoprotein B production rate was 0.70 +/- 0.18 mg X kg-1 X h-1. Comparison of the total low-density lipoprotein apoprotein B production with that derived from very-low-density lipoprotein apoprotein B suggested that a large fraction of plasma low-density lipoprotein apoprotein B was derived from a source exclusive of circulating very-low-density lipoprotein apoprotein B. This was confirmed in two animals by simultaneous injection of radiolabeled very-low-density and low-density lipoproteins. Thus, a significant proportion of cynomolgus monkey low-density lipoproteins are produced either by direct hepatic secretion or by rapid conversion of lower-density lipoproteins before they appear in the peripheral circulation.