A protocol for the differentiation of human embryonic stem cells into dopaminergic neurons using only chemically defined human additives: Studies in vitro and in vivo
A protocol for the differentiation of human embryonic stem cells into dopaminergic neurons using only chemically defined human additives: Studies in vitro and in vivo
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DOI：
10.1016/j.brainres.2006.10.022
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发表时间：
2007-01-05
期刊：
影响因子：
2.9
通讯作者：
Yang, Ming
中科院分区：
文献类型：
--
作者：
Iacovitti, Lorraine;Donaldson, Angela E.;Yang, Ming
我们能否利用人类胚胎干细胞（hES）进行帕金森病的细胞替代疗法，取决于能否找到简单、可靠地分化这些细胞中的多巴胺能（DA）表型的方法。虽然有几种方案可用于he中DA性状的分化，但它们涉及长期使用成分不明的复杂培养基，细胞条件培养基和/或与各种细胞共培养，通常来自动物。在本研究中，使用一种仅使用化学定义的人源性培养基添加剂和底物的新型快速方案，研究了几种具有良好特征的（H9, BG01）和几种新的未表征的（HUES7, HUES8） hES细胞系在培养中分化为DA神经元的能力。在3周内，4种细胞系的细胞从未分化状态进展为体外表达DA标记物的β -微管蛋白III阳性细胞。此外，将这些细胞移植到6-羟多巴胺处理的大鼠神经元祖细胞期纹状体中，2-3周后在体内出现分化的DA特征。(c) 2006 Elsevier B.V.版权所有
Our ability to use human embryonic stem (hES) cells in cell replacement therapy for Parkinson's disease depends on the discovery of ways to simply and reliably differentiate a dopaminergic (DA) phenotype in these cells. Although several protocols exist for the differentiation of DA traits in hES, they involve the prolonged use of complex media with undefined components, cell conditioned media and/or co-culture with various cells, usually of animal origin. In this study, several well-characterized (H9, BG01) and several new uncharacterized (HUES7, HUES8) hES cell lines were studied for their capacity to differentiate into DA neurons in culture using a novel rapid protocol which uses only chemically-defined human-derived media additives and substrata. Within 3 weeks, cells from all 4 cell lines progressed from the undifferentiated state to beta-tubulin III positive cells expressing DA markers in vitro. Moreover, transplantation of these cells into the striata of 6-hydroxydopamine-treated rats at the neuronal progenitor stage resulted in the appearance of differentiated DA traits in vivo 2-3 weeks later. (c) 2006 Elsevier B.V. All rights reserved.