DENDRITIC ALTERATION OF RAT SPINAL MOTONEURONS AFTER DORSAL HORN MINCE - COMPUTER RECONSTRUCTION OF DENDRITIC FIELDS
DENDRITIC ALTERATION OF RAT SPINAL MOTONEURONS AFTER DORSAL HORN MINCE - COMPUTER RECONSTRUCTION OF DENDRITIC FIELDS
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DOI：
10.1016/0014-4886(83)90078-x
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发表时间：
1983-01-01
影响因子：
5.3
通讯作者：
STANDLER, NA
中科院分区：
文献类型：
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作者：
BERNSTEIN, JJ;STANDLER, NA
哺乳动物脊髓运动神经元树突在腹根挤压后会出现周期性变性和再生。切碎背角后，分析大鼠 IX 板、内侧、[胸椎] T2 椎骨下运动神经元的横截面树突轮廓（正常，术后 14、30、60、90 天 (DPO)；n = 6 只动物/DPO）。通过高尔基体技术的钨酸盐改良来浸渍脊髓。通过编码载玻片测量沿着分支点之间的树突段的各个长度，将数据进行计算机化并通过计算机重建树突。通过 ANOVA（方差分析）先验和 Newman-Keuls 后验检验进行动物间统计比较。在 DPO 14 时，与正常完整大鼠和术后所有其他天相比，树突节段数量、树突和蛇形长度以及从躯体发出的节段数量有统计学显着性（P < 0.05）增加。在 30 和 60 DPO 时，这些参数恢复到正常值；然而，有许多长的、无分支的树突。在 90 DPO 时，运动神经元树突蛇形长度和节段出现统计学显着性下降（P < 0.05）。部分传入神经缺失的大鼠脊髓运动神经元经历双相反应；初始生长阶段，随后是退化阶段。
Mammalian spinal motoneuron dendrites respond with cyclic degeneration and regeneration after ventral root crush. The cross-sectional dendritic profile of rat lamina IX, medial, motoneurons under the [thoracic] T2 vertebra were analyzed after mincing the dorsal horn (normals, 14, 30, 60, 90 days postoperative (DPO); n = 6 animals/DPO). The spinal cords were impregnated by the tungstate modification of the Golgi technique. Individual lengths along dendritic segments between branching points were measured from coded slides, the data were computerized and the dendrites were reconstructed by computer. Interanimal statistical comparisons were made by ANOVA [analysis of variance] a priori and Newman-Keuls test a posteriori. At 14 DPO, there was a statistically significant (P < 0.05) increase in the number of dendritic segments, dendritic and serpentine length and number of segments emanating from the soma compared with normal intact rats and with all other postoperative days. At 30 and 60 DPO, these parameters returned to normal values; however, there were many long, unbranched dendrites. At 90 DPO, there was a statistically significant (P < 0.05) decrease in motoneuron dendritic serpentine length and segments. Partially deafferented rat spinal motoneurons undergo a biphasic response; an initial growth phase followed by a degenerative phase.