Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A
Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A
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DOI：
10.1016/j.virol.2016.07.003
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发表时间：
2016-10-01
期刊：
影响因子：
3.7
通讯作者：
Fang, Ying
中科院分区：
文献类型：
--
作者：
Chen, Zhenhai;Yuan, Fangfeng;Fang, Ying
利用新出现的塞内卡病毒A （SVA；株KS15-01）构建了全长cDNA感染性克隆pKS15-01-Clone。为了探索作为表达标记基因的病毒骨干的潜力，利用反向遗传学方法生成了增强型绿色荧光蛋白（EGFP）标记的报告病毒（vKS15-01-EGFP）。与亲本病毒相比，pKS15-01-Clone衍生病毒（vKS15-01-Clone）在体外和体内均能高效复制，并在感染动物体内诱导相似水平的中和抗体和细胞因子反应。相比之下，vKS15-01-EGFP病毒在感染动物中表现出生长能力受损和诱导较低的免疫应答水平。所有感染亲本病毒KS15-01的猪鼻背和冠状带均出现病变，而感染vKS15-01-Clone和vKS15-01-EGFP病毒的猪没有出现病变。这些结果表明，感染克隆和EGFP报告病毒可作为进一步阐明SVA发病机制和制定控制措施的重要工具。(C) 2016 Elsevier Inc.版权所有。
A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary bands were observed in all pigs infected by parental virus KS15-01, but not in pigs infected with vKS15-01-Clone or vKS15-01-EGFP viruses. These results demonstrated that the infectious clone and EGFP reporter virus could be used as important tools in further elucidating the SVA pathogenesis and development of control measures. (C) 2016 Elsevier Inc. All rights reserved.