Empowering engineered muscle in biohybrid pump by extending connexin 43 duration with reduced graphene oxides
Empowering engineered muscle in biohybrid pump by extending connexin 43 duration with reduced graphene oxides
复制标题
通过减少氧化石墨烯延长连接蛋白 43 的持续时间，增强生物混合泵中的工程肌肉
DOI：
10.1016/j.biomaterials.2022.121643
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发表时间：
2022
期刊：
影响因子：
14
通讯作者：
Kong, Hyunjoon
中科院分区：
文献类型：
--
作者：
Ko, Eunkyung;Aydin, Onur;Li, Zhengwei;Gapinske, Lauren;Huang, Kai-Yu;Saif, Taher;Bashir, Rashid;Kong, Hyunjoon
工程化骨骼肌作为治疗受伤或患病肌肉的宝贵治疗剂和组装生物机械所必需的“活”材料。对于正常发育，骨骼肌成肌细胞应在早期分化阶段表达连接蛋白43，其为促进成肌细胞融合和肌生成的差距连接蛋白之一。然而，体外培养的成肌细胞通常在分化前下调连接蛋白43，限制肌生成和肌肉收缩。这项研究表明，用还原氧化石墨烯（rGO）束缚成肌细胞减缓了早期分化过程中连接蛋白43的消退，并增加了肌源性mRNA的合成。全RNA测序还证实，细胞上的rGO增加了肌生成的调节基因，包括肌钙蛋白，同时减少了负调节基因。所得到的肌管产生比无rGO的肌管大三倍的收缩力。因此，与rGO栓系肌肉组装的无阀生物混合泵显著增加了流体速度和流速。这项研究的结果将为开发生理相关的肌肉和为生物机器提供动力提供重要的基础，这些生物机器将用于各种生物科学研究和未开发的应用。
Engineered skeletal muscle act as therapeutics invaluable to treat injured or diseased muscle and a “living” material essential to assemble biological machinery. For normal development, skeletal myoblasts should express connexin 43, one of the gap junction proteins that promote myoblast fusion and myogenesis, during the early differentiation stage. However, myoblasts cultured in vitro often down-regulate connexin 43 before differentiation, limiting myogenesis and muscle contraction. This study demonstrates that tethering myoblasts with reduced graphene oxide (rGO) slows connexin 43 regression during early differentiation and increases myogenic mRNA synthesis. The whole RNA sequencing also confirms that the rGO on cells increases regulator genes for myogenesis, including troponin, while decreasing negative regulator genes. The resulting myotubes generated a three-fold larger contraction force than the rGO-free myotubes. Accordingly, a valveless biohybrid pump assembled with the rGO-tethered muscle increased the fluid velocity and flow rate considerably. The results of this study would provide an important foundation for developing physiologically relevant muscle and powering up biomachines that will be used for various bioscience studies and unexplored applications.