STRUCTURAL STUDIES ON THE PHOSPHATE-FREE LIPID-A OF RHODOMICROBIUM-VANNIELII ATCC-17100
STRUCTURAL STUDIES ON THE PHOSPHATE-FREE LIPID-A OF RHODOMICROBIUM-VANNIELII ATCC-17100
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DOI：
10.1111/j.1432-1033.1983.tb07832.x
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
MAYER, H
中科院分区：
文献类型：
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作者：
HOLST, O;BOROWIAK, D;MAYER, H
本文报道了R. vannielii ATCC 17，100的菌株。它由一个中央β-未被磷酸取代的1“，6-连接的葡糖胺二糖。约30%的二糖分子被β-吡喃甘露糖取代。与非还原性葡糖胺的1，4“-连接。还原性葡糖胺可以用NaBH 4直接还原，表明该葡糖胺在C1处未被取代，或者其取代基在游离脂质A的制备过程中被除去，或者在用NaBH 4还原的过程中被除去。下式显示了来自R的游离脂质A的骨架结构。vannielii ATCC 17，100：β- Manp（1.sbd.-→4）-β- GlcpN（1 →6）GlcpN。3-（R）-羟基十六烷酸与还原性葡糖胺的氨基连接。未鉴别出非还原氨基葡萄糖的氨基残基。中心二糖的羟基被3-（十四酰氧基）-十四烷酸、3-羟基十四烷酸、Δ 14-二十二碳烯酸（Δ 14-C22：1）和乙酰基酰化。甘露糖的羟基未被取代。
The structure of the free lipid A from R. vannielii ATCC 17,100 was elucidated. It consists of a central .beta.-1'',6-linked glucosamine disaccharide which is not substituted by phosphate. About 30% of the disaccharide molecules are substituted with mannopyranose in .beta.-1,4''-linkage to the non-reducing glucosamine. The reducing glucosamine can be directly reduced with NaBH4, indicating that this glucosamine is not substituted at C1 or its substituent was removed during the preparation of free lipid A or is removed during reduction with NaBH4. The following formula shows the backbone structure of the free lipid A from R. vannielii ATCC 17,100: .beta.-Manp(1.sbd.- .fwdarw. 4)-.beta.-GlcpN(1 .fwdarw. 6)GlcpN. 3-(R)-Hydroxyhexadecanoic acid is linked to the amino group of the reducing glucosamine. The residue at the amino group of the non-reducing glucosamine was not identified. The hydroxyl groups of the central disaccharide are acylated with 3-(tetradecanoyloxy)-tetradecanoic acid, 3-hydroxytetradecanoic acid, .DELTA.14-docosenoic acid (.DELTA.14-C22:1) and acetyl groups. The hydroxyl groups of the mannose are not substituted.