On-line control of feeding of medium components to attain high cell density
On-line control of feeding of medium components to attain high cell density
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DOI：
10.1007/s004490050756
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发表时间：
2000-05
期刊：
影响因子：
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通讯作者：
H. Suzuki;M. Kishimoto;Y. Kamoshita;T. Omasa;Y. Katakura;K. Suga
中科院分区：
文献类型：
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作者：
H. Suzuki;M. Kishimoto;Y. Kamoshita;T. Omasa;Y. Katakura;K. Suga
在线控制与专家系统相结合，用于控制流加培养，目的是实现高细胞密度。在培养过程中，专家系统可以每10分钟监测培养基中化学元素量的波动程度，并且如果特定组分的量不足以用于细胞生长，则建议对补料控制策略进行修改。然而，我们经常遇到这样的一类培养，其中某些特定的碳源，如葡萄糖和乙醇，在许多培养过程中应控制在低浓度，因为过量的补料可能会导致生长抑制，通过其自身的积累在培养液中，或副产物的积累，如乳酸盐或乙酸盐，不适合平稳生长的物质。在这项研究中，我们开发了一个在线控制系统的基础上产生式规则管理葡萄糖进料速率从DO信号。由于专家系统的推理需要较长的时间（几分钟），而碳源饥饿对细胞生长的影响即使在几分钟内也是不可忽略的，因此通过控制计算机与专家系统的另一台计算机相连进行在线控制。将专家系统在线控制系统应用于大肠杆菌细胞团的生产。coliW 3110，最终菌体浓度达到137 g-菌体干重/l。
On line control coupled with an expert system was constructed for the control of a fed-batch culture with the aim of achieving a high cell density. During the cultivation, the expert system could monitor the extents of sufficiencies in the amounts of chemical elements in the medium every 10 min, and suggest a modification to the feeding control policy if the amount of a particular component was inadequate for cell growth. However, we often encounter such a kind of cultivations in which some particular carbon source such as glucose and ethanol should be controlled at low concentration in many culture processes, because the excess feeding might cause the growth inhibition by its own accumulation in the culture broth, or the biproduct accumulation like as lactate or acetate, unsuitable substances for smoothed growth. In this study, we developed an online control system based on production rules which managed the glucose feed rate from DO signal. The online control was carried out by the control computer connected with the other computer for expert system, because a relatively long time (several minutes) was needed for the inference of the expert system and the influence of the starvation of carbon source on the cell growth is not negligible even in several minutes. The on-line control system with expert system was applied to the production of cell mass ofE. coliW3110 and the final cell concentration reached 137 g-dry cell weight/l.