Protocol for the generation of murine bronchiolospheres.
Protocol for the generation of murine bronchiolospheres.
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DOI：
10.1016/j.xpro.2021.100594
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发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
El Agha E
中科院分区：
文献类型：
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作者：
Vazquez-Armendariz AI;Seeger W;Herold S;El Agha E
类器官模型已被证明是研究生物和病理环境中上皮间质串扰的有价值的工具。我们的数据确定 ACTA2+ PDGFRα+ 修复支持间充质细胞是传导气道生态位的重要组成部分。在这里，我们提供了培养气道类器官或细支气管球的详细方案，它可以评估间充质细胞支持俱乐部细胞生长的能力。有关该协议的使用和执行的完整详细信息，请参阅。细支气管球是模拟上皮-间质相互作用的有用工具。不同类型的间充质细胞可用于支持俱乐部细胞生长。培养 16 天后即可评估细支气管内的细胞分化。细支气管球可以传代多次。类器官模型已被证明是研究生物和病理环境中上皮-间质相互作用的宝贵工具。我们的数据确定 ACTA2+ PDGFRα+ 修复支持间充质细胞 (RSMC) 是传导气道生态位的重要组成部分。在这里，我们提供了培养气道类器官或细支气管球的详细方案，它可以评估间充质细胞支持俱乐部细胞生长的能力。
Organoid models have been shown to be valuable tools for studying epithelial-mesenchymal crosstalk during biological and pathological settings. Our data identified ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the conducting airway niche. Here, we provide a detailed protocol for culturing airway organoids, or bronchiolospheres, which provide an assessment of the ability of mesenchymal cells to support club-cell growth. For complete details on the use and execution of this protocol, please refer to. Bronchiolospheres are a useful tool to model epithelial-mesenchymal interactions Different types of mesenchymal cells can be used to support club cell growth Cell differentiation within bronchiolospheres can be assessed after 16 days of culture Bronchiolospheres can be passaged multiple times Organoid models have been shown to be valuable tools for studying epithelial-mesenchymal crosstalk during biological and pathological settings. Our data identified ACTA2+ PDGFRα+ repair-supportive mesenchymal cells (RSMCs) as an important component of the conducting airway niche. Here, we provide a detailed protocol for culturing airway organoids, or bronchiolospheres, which provide an assessment of the ability of mesenchymal cells to support club-cell growth.