Measurement of oxysterols and α-tocopherol in plasma and tissue samples as indices of oxidant stress status
Measurement of oxysterols and α-tocopherol in plasma and tissue samples as indices of oxidant stress status
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DOI：
10.1016/s0003-2697(02)00467-0
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发表时间：
2003-01-15
影响因子：
2.9
通讯作者：
Diczfalusy, U
中科院分区：
文献类型：
--
作者：
Iuliano, L;Micheletta, F;Diczfalusy, U
氧化应激似乎在人类病理学的几种情况下发挥作用，例如动脉粥样硬化、癌症和衰老。人类疾病中氧化应激的研究应基于通过测量α-生育酚来评估增强的氧化应激的敏感和特异性标志物，如氧化固醇或抗氧化防御。我们已经开发了一种快速的方法来测量血浆（50名健康受试者）和组织中的氧化固醇7 β-羟基胆固醇和7-酮胆固醇作为体内氧化应激的指标，并从相同的样品α-生育酚含量。7 β-羟基胆固醇和7-酮胆固醇的平均血浆浓度分别为4.6 +/- 1.1和13.4 +/- 7.6 ng/mL。血浆α-生育酚浓度为5.8 +/- 1.0 mumol/mol胆固醇。与正常动脉相比，动脉粥样硬化斑块的样本含有20倍多的胆固醇，约45倍高的氧化固醇水平和600倍多的α-生育酚。胆固醇和氧固醇含量在正常肝和慢性肝病肝中无显著性差异。然而，与正常肝脏相比，异位肝脏含有显著更低浓度的α-生育酚。总之，我们已经开发了一种快速可靠的方法，用于测定血浆和组织中的胆固醇氧化产物和α-生育酚，可用于估计氧化应激/抗氧化剂平衡。(C)2003 Elsevier Science（美国）。All rights reserved.
Oxidant stress seems to play a role in several setting of human pathology, such as atherosclerosis, cancer, and aging. The study of oxidant stress in human disease should be based on the evaluation of either sensitive and specific markers of enhanced oxidant stress, such as oxysterols, or antioxidant defense, by measuring alpha-tocopherol. We have developed a rapid method to measure the oxysterols 7beta-hydroxycholesterol and 7-ketocholesterol in plasma (50 healthy subjects) and tissue as an index of oxidant stress in vivo, and from the same sample alpha-tocopherol content. The mean plasma concentration of 7beta-hydroxycholesterol and 7-ketocholesterol was 4.6 +/- 1.1 and 13.4 +/- 7.6 ng/mL, respectively. Plasma alpha-tocopherol concentration was 5.8 +/- 1.0 mumol/mol cholesterol. Samples from atherosclerotic plaques contained 20 times more cholesterol, about 45 times higher oxysterols levels, and 600 times more alpha-tocopherol compared to normal arteries. No significant difference in cholesterol and oxysterol content was observed between cirrhotic and normal liver. However, cirrhotic liver contained significantly smaller concentration of alpha-tocopherol compared to normal liver. In conclusion, we have developed a rapid and reliable method for the assay of cholesterol oxidation products and alpha-tocopherol in plasma and tissue useful for estimation of oxidant stress/antioxidant balance. (C) 2003 Elsevier Science (USA). All rights reserved.