The transcription factors HvABI5 and HvVP1 are required for the abscisic acid induction of gene expression in barley aleurone cells
The transcription factors HvABI5 and HvVP1 are required for the abscisic acid induction of gene expression in barley aleurone cells
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DOI：
10.1105/tpc.007096
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发表时间：
2003-01-01
期刊：
影响因子：
11.6
通讯作者：
Ho, THD
中科院分区：
文献类型：
--
作者：
Casaretto, J;Ho, THD
脱落酸（阿坝）反应启动子复合物（ABRC）的HVA 1和HVA 22基因已被证明赋予ABA诱导的基因表达谷物。大麦碱性结构域/亮氨酸拉链（bZIP）转录因子，HvAB 15，能够识别ABRC在体外以序列特异性的方式和反式激活ABRC-β-葡萄糖醛酸酶报告基因时，引入大麦糊粉细胞通过粒子轰击。这种反式激活依赖于另一种转录因子HvVP 1的存在，并且不能被负调节因子abi 1 -1阻断。使用双链RNA干扰技术，我们表明，HvAB 15和HvVP 1是必需的阿坝诱导的基因表达，但没有影响另一个受ABA调节的过程，赤霉素诱导和ABA抑制的α-淀粉酶的表达。我们的工作表明，虽然其他典型的植物bZIP转录因子可以结合ABRCs在体外，HvAB 15是相关的一个亚家族的bZIP负责阿坝诱导的基因表达。此外，HvAB 15和HvVF 1不参与阿坝对基因表达的抑制。
The abscisic acid (ABA) response promoter complexes (ABRCs) of the HVA1 and HVA22 genes have been shown to confer ABA-induced gene expression in cereals. A barley basic domain/Leu zipper (bZIP) transcription factor, HvAB15, is able to recognize ABRCs in vitro in a sequence-specific manner and to transactivate ABRC-beta-glucuronidase reporter genes when introduced to barley aleurone cells via particle bombardment. This transactivation is dependent on the presence of another transcription factor, HvVP1, and cannot be blocked by the negative regulator abi1-1. Using the double-stranded RNA interference technique, we show that HvAB15 and HvVP1 are necessary for the ABA induction of gene expression but have no effect on another hormone-regulated process, the gibberellin-induced and ABA-suppressed expression of alpha-amylase. Our work indicates that although other typical plant bZIP transcription factors may bind ABRCs in vitro, HvAB15 is related to a subfamily of bZIPs responsible for the ABA induction of gene expression. Furthermore, HvAB15 and HvVF1 are not involved in the ABA suppression of gene expression.