Overproduction of three genes leads to camphor resistance and chromosome condensation in Escherichia coli.
Overproduction of three genes leads to camphor resistance and chromosome condensation in Escherichia coli.
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发表时间：
1996-08
期刊：
影响因子：
3.3
通讯作者：
H. Kenneth;Esmeralda;Liu;Keith Dean;Gingas;William DeGrafft;N. Trun
中科院分区：
文献类型：
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作者：
H. Kenneth;Esmeralda;Liu;Keith Dean;Gingas;William DeGrafft;N. Trun
我们分离并表征了三个基因，crcA，cspE和crcB，当其以高拷贝存在时赋予细胞樟脑抗性并抑制染色体分区基因mukB中的突变。两种表型都需要相同的基因。与染色体樟脑抗性突变体不同，高拷贝数crcA、cspE和crcB不会导致细胞倍性的增加。cspE基因先前已被鉴定为冷休克样蛋白，在所有测试的生物体中具有同源物。我们还表明，樟脑导致的类核在体内deconcense，当这三个基因存在于高拷贝，染色体不deconcense。我们的研究结果暗示樟脑和mukB突变干扰染色体凝聚和高拷贝crcA，cspE和crcB作为促进或保护染色体折叠。
We isolated and characterized three genes, crcA, cspE and crcB, which when present in high copy confer camphor resistance on a cell and suppress mutations in the chromosomal partition gene mukB. Both phenotypes require the same genes. Unlike chromosomal camphor resistant mutants, high copy number crcA, cspE and crcB do not result in an increase in the ploidy of the cells. The cspE gene has been previously identified as a cold shock-like protein with homologues in all organisms tested. We also demonstrate that camphor causes the nucleoids to decondense in vivo and when the three genes are present in high copy, the chromosomes do not decondense. Our results implicate camphor and mukB mutations as interfering with chromosome condensation and high copy crcA, cspE and crcB as promoting or protecting chromosome folding.