Lectin-resistant CHO cells: selection of four new pea lectin-resistant phenotypes.
Lectin-resistant CHO cells: selection of four new pea lectin-resistant phenotypes.
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凝集素抗性 CHO 细胞：四种新的豌豆凝集素抗性表型的选择。
DOI：
10.1007/bf01560727
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Stanley,P
中科院分区：
文献类型：
--
作者：
Ripka,J;Stanley,P
从P. sativum已经被用于从中国仓鼠卵巢（CHO）细胞中选择新的凝集素抗性突变体。两个新的表型，表现recessions，但属于同一互补组已被称为Lec 13和Lec 13 A。这两种突变类型在l-岩藻糖中生长后表型回复为豌豆凝集素敏感性。相反，另外两种独特的表型在体细胞杂种中占主导地位，并在岩藻糖存在下保持其豌豆凝集素抗性。它们分别被称为LEC 14和LEC 18。四种新突变类型的凝集素抗性和互补特性表明它们定义了CHO基因组的三种不同糖基化基因。
The cytotoxic plant lectins fromP. sativumhave been used to select new lectin-resistant mutants from Chinese hamster ovary (CHO) cells. Two novel phenotypes that behave recessively but fall into the same complementation group have been termed Lec 13 and Lec 13A. Both of these mutant types are phenotypically reverted to pea lectin sensitivity following growth inl-fucose. In contrast, two other unique phenotypes behave dominantly in somatic cell hybrids and maintain their pea lectin-resistance in the presence ofl-fucose. They have been termed LEC14 and LEC18, respectively. The lectin-resistance and complementation properties of the four new mutant types suggest that they define three different glycosylation genes of the CHO genome.