Proteome profile of maize (Zea Mays L.) leaf tissue at the flowering stage after long-term adjustment to rice black-streaked dwarf virus infection
Proteome profile of maize (Zea Mays L.) leaf tissue at the flowering stage after long-term adjustment to rice black-streaked dwarf virus infection
复制标题
水稻黑条矮缩病毒感染长期调整后开花期玉米（Zea Mays L.）叶组织的蛋白质组谱
DOI：
10.1016/j.gene.2011.06.016
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发表时间：
2011-10-10
期刊：
影响因子：
3.5
通讯作者：
Zhang, Juren
中科院分区：
文献类型：
--
作者：
Li, Kunpeng;Xu, Changzheng;Zhang, Juren
玉米粗缩病是一种病毒性病害，严重影响玉米产量.为了更好地了解MRDD对植物生长和代谢的影响，对来自病毒感染和正常植物的叶片进行了比较蛋白质组学分析。为了消除核酮糖-1，5-二磷酸羧化酶对低丰度蛋白质的干扰，采用15%PEG对总蛋白质进行预分级，然后对上清液和沉淀组分进行双向电泳分析。在检测到的约1200个蛋白质斑点中，凝胶上少于2%的斑点在沉淀和上清液的级分之间重叠。我们确定了91个差异积累的蛋白质，属于多个代谢/生化途径在植物中。对这些蛋白质的进一步分析表明，MRDD导致了糖酵解和淀粉代谢等基本代谢的显著变化，并最终导致了感染病毒和正常植物之间在形态和发育方面的显著差异。此外，MRDD的发生增加了对G蛋白、抗氧化酶、脂氧合酶和UDP-葡萄糖基转移酶BX 9的需求，这些酶在植物抗病毒感染的反应中可能起重要作用。MRDD是一种多基因参与的复杂疾病。(C)2011 Elsevier B. V.保留所有权利。
Maize rough dwarf disease (MRDD) is a viral disease and causes great yield loss. To better understand the effects of MRDD on plant growth and metabolism, comparative proteomic analysis of leaves from virus-infected and normal plants was performed. In order to eliminate the interference of Ribulose-1, 5-bisphosphate carboxylase with low-abundance proteins, total proteins were pre-fractionated by 15% PEG and the proteins from supernatant and precipitated fractions were analyzed by 2-DE, subsequently. Out of approximately 1200 protein spots detected, less than 2% of the spots on the gels were overlapping between the fractions of precipitation and supernatant. We identified 91 differentially accumulated proteins that belong to multiple metabolic/biochemical pathways in plants. Further analysis of these identified proteins indicated that MRDD resulted in dramatic changes in the fundamental metabolism, including glycolysis and starch metabolism, and eventually the significant differences in morphology and development between virus-infected and normal plants. Moreover, MRDD occurrence increased the demands for G-proteins, antioxidant enzymes, lipoxygenases and UDP-glucosyltransferase BX9, which may play important roles in response of plant against virus infection. The results also suggested that MRDD is a complicated disease controlled by multigene participating in different pathways. (C) 2011 Elsevier B.V. All rights reserved.