A RECOVERED AVIAN MYELOCYTOMATOSIS VIRUS THAT INDUCES LYMPHOMAS IN CHICKENS - PATHOGENIC PROPERTIES AND THEIR MOLECULAR-BASIS
A RECOVERED AVIAN MYELOCYTOMATOSIS VIRUS THAT INDUCES LYMPHOMAS IN CHICKENS - PATHOGENIC PROPERTIES AND THEIR MOLECULAR-BASIS
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DOI：
10.1016/0092-8674(83)90170-8
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
HAYMAN, MJ
中科院分区：
文献类型：
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作者：
ENRIETTO, PJ;PAYNE, LN;HAYMAN, MJ
禽髓细胞瘤病毒MC29通过v-myc基因的作用可引起鸡的骨髓瘤和肾、肝肿瘤等多种肿瘤。MC29从未被报道会导致淋巴样肿瘤，这种疾病与c-myc基因通过插入淋巴白血病病毒基因组而激活有关。对回收的MC29病毒HBL进行了序列分析，该病毒含有一个含有c-myc序列的myc基因和一些v-myc序列。将HBL接种到鸡体内，可导致与法氏囊无关的淋巴样肿瘤。在抗原性方面，这些肿瘤由T细胞和B细胞组成。分子分析显示，39例肿瘤中有36例HBL前病毒DNA，c-myc无明显变化，肿瘤细胞中可检测到HBL gag-myc融合蛋白p108。根据myc基因转化靶细胞专一性的机制对这些数据进行了讨论。
The avian myelocytomatosis virus MC29 induces neoplastic diseases in chicken, including myelocytomas and tumors of kidney and liver, which are due to the action of the v-myc gene. MC29 has never been reported to cause lymphoid tumors, the disease associated with activation of the c-myc gene by the insertion of a lymphoid leukosis virus genome. A recovered MC29 virus, HBl, which has a myc gene containing c-myc sequences, acquired by recombination with the cellular gene, and some v-myc sequences was analyzed. Inoculation of HBl into chickens resulted in lymphoid tumors independent of the bursa. Antigenically these tumors were made up of T and B cells. Molecular analysis showed HBl proviral DNA in 36 of 39 tumors analyzed, with no obvious alteration of c-myc, and the HBl gag-myc fusion protein, p108, could be detected in tumor cells. These data are discussed in terms of the mechanism of target-cell specificity for transformation by the myc gene.