A monoclonal antibody detecting unusual Thy-1 determinants.
A monoclonal antibody detecting unusual Thy-1 determinants.
复制标题
检测异常 Thy-1 决定簇的单克隆抗体。
作者：
H. Auchincloss;K. Ozato;D. Sachs
2/0 -10-5S是C3 H抗C3 H.SW脾细胞与SP2/0细胞系融合产生的单克隆抗体。该抗体似乎与Thy-1决定簇反应的几个标准，包括细胞毒性模式，功能测定，遗传分析，和竞争性结合实验。然而，其检测到的抗体和决定簇是不寻常的，因为：1）20-10-5S是自身反应性的; 2）抗体仅在外周淋巴细胞上而不是在胸腺细胞上显示对Thy-1.2与Thy-1.1抗原的同种特异性; 3）抗体仅与鼠T细胞上的决定簇反应，而不是与脑组织或大鼠胸腺细胞上的抗原反应。因此，似乎20-10-5S与鼠T细胞特异性Thy-1决定簇反应，这些决定簇在表达它们的细胞成熟期间丢失或修饰。
20-10-5S is a monoclonal antibody produced by the fusion of C3H anti-C3H.SW splenocytes with the SP2/0 cell line. The antibody appears to react with Thy-1 determinants by several criteria including cytotoxicity patterns, functional assays, genetic analyses, and competitive binding experiments. However, the antibody and the determinants it detects are unusual in that: 1) 20-10-5S is autoreactive; 2) the antibody shows allospecificity for Thy-1.2 vs Thy-1.1 antigens only on peripheral lymphocytes and not on thymocytes; and 3) the antibody reacts only with determinants on murine T cells and not with antigens on brain tissue or on rat thymocytes. It therefore seems that 20-10-5S reacts with murine T cell-specific Thy-1 determinants that are lost or modified during maturation of the cells on which they are expressed.