Development of a 3D cell culture system for investigating cell interactions with electrospun fibers
Development of a 3D cell culture system for investigating cell interactions with electrospun fibers
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DOI：
10.1002/bit.21309
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发表时间：
2007-08-01
影响因子：
3.8
通讯作者：
MacNeil, Sheila
中科院分区：
文献类型：
--
作者：
Sun, Tao;Norton, David;MacNeil, Sheila
在研究细胞如何与组织工程中使用的3D支架相互作用时，需要考虑许多变量。本研究探讨了纤维支架对人真皮成纤维细胞行为的影响。两种不同的模型材料，聚苯乙烯和聚-L-乳酸，具有广泛的直径范围内的纤维构建在一个专门开发的三维细胞培养系统。当成纤维细胞被引入到独立的纤维，并鼓励“走木板”，观察到细胞粘附和迁移的最小纤维直径为10 μ m，与纤维材料的化学性质无关。纤维之间的距离高达200 μ m，也被观察到的最大间隙，可以通过细胞聚集体桥接-在传统的二维培养中没有看到的行为。这种方法已经确定了电纺支架设计的一些基本微结构参数和3D成纤维细胞生长的一些关键差异。我们认为，这些发现将是有价值的优化整合细胞在这些支架的皮肤组织工程。
There are many variables to be considered in studying how cells interact with 3D scaffolds used in tissue engineering. In this study we investigated the influence of the fiber scaffolds on the behaviors of human dermal fibroblasts. Fibers of two dissimilar model materials, polystyrene and poly-L-Lactic acid, with a broad range of diameters were constructed in a specifically developed 3D cell culture system. When fibroblasts were introduced to freestanding fibers, and encouraged to "walk the plank", a minimum fiber diameter of 10 mu m was observed for cell adhesion and migration, irrespective of fiber material chemistry. A distance between fibers of up to 200 mu m was also observed to be the maximum gap that could be bridged by cell aggregates - a behavior not seen in conventional 2D culture. This approach has identified some basic micro-architectural parameters for electrospun scaffold design and some key differences in fibroblast growth in 3D. We suggest the findings will be of value for optimizing the integration of cells in these scaffolds for skin tissue engineering.