Allelic loss in childhood acute lymphoblastic leukemia
Allelic loss in childhood acute lymphoblastic leukemia
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DOI：
10.1016/s0145-2126(97)00075-1
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发表时间：
1997-09-01
影响因子：
2.7
通讯作者：
Sinnett, D
中科院分区：
文献类型：
--
作者：
Baccichet, A;Qualman, SK;Sinnett, D
急性淋巴细胞白血病(ALL)是儿童最常见的癌症。对儿童ALL的分子基础知之甚少，尽管其临床、病理和免疫表型特征已有很好的文献记载。为了了解肿瘤抑制基因(TSG)在该病发生发展中的作用，我们进行了详细的等位基因分析。对29例患者(24例前B细胞和5例T细胞系)进行了杂合性丢失(LOH)调查，使用了分布在13条染色体臂上的49个高度多态的标记，这些染色体上已知或推测含有TSG。在有信息的患者中，9p和12p的等位基因缺失率最高，分别为29和32%。这些都是儿童时期最常见的变化之一。在染色体6p、6q、9q、17p和17q上发现了其他频率较低的缺失。在染色体3p、5q、11p、11q、13q和18q上均未发现杂合性缺失。这些结果表明，许多TSG可能参与了儿童ALL的发展。(C)1997年爱思唯尔科学有限公司。
Acute lymphoblastic leukemia (ALL) is the most frequent cancer encountered in children. Little is known about the molecular basis of childhood ALL, although the clinical, pathological, and immunophenotypic features have been well documented. To understand the role of tumor suppressor genes (TSGs) in the development of this disease, we performed a detailed allelotype analysis. Twenty-nine patients (24 pre-B and 5 of T-cell lineage) were investigated for loss of heterozygosity (LOH), using 49 highly polymorphic markers distributed over 13 chromosomal arms which are known or postulated to contain TSGs. The highest rates of allelic losses were observed in chromosomes 9p and 12p which were deleted in 29 and 32% of the informative patients, respectively. These are among the most frequent alterations found in childhood ALL. Other losses were found at a lower frequency in chromosomes 6p, 6q, 9q, 17p, and 17q. No LOH was found at chromosomes 3p, 5q, 11p, 11q, 13q and 18q in any patient. These results suggest that many TSGs may be involved in the development of childhood ALL. (C) 1997 Elsevier Science Ltd.