Comparison of PCR and culture for detection of Nocardia asteroides in brain specimens from experimentally infected BALB/c mice.
Comparison of PCR and culture for detection of Nocardia asteroides in brain specimens from experimentally infected BALB/c mice.
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PCR 和培养物检测实验感染 BALB/c 小鼠脑标本中小行星诺卡氏菌的比较。
DOI：
10.1016/j.micres.2004.05.003
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
LeWitt,PeterA
中科院分区：
文献类型：
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作者：
Loeffler,DavidA;Camp,DianneM;Nichols,LihuaQu;Maksaereekul,Saipiroon;Beaman,BlaineL;LeWitt,PeterA
用星状诺卡氏菌菌株GUH-2全身感染BALB/c小鼠导致该生物体在脑中广泛复制，随后其免疫介导的清除。本研究比较了聚合酶链反应（PCR）和细菌培养检测脑梗死的敏感性。小鼠（n=4/时间点）给予N.在感染后第2、7、14和21天，通过PCR和培养评价脑标本中的诺卡氏菌。通过PCR在感染后第2、7和14天的所有感染动物中检测到诺卡氏菌，在感染后第21天的4只小鼠中的1只中检测到诺卡氏菌;相反，仅在感染后第2和7天通过培养检测到该微生物。这些发现表明，PCR可能比培养更敏感，用于检测脑中的低数量诺卡氏菌。
Systemic infection of BALB/c mice with Nocardia asteroides strain GUH-2 results in widespread replication of the organism in the brain, followed by its immune-mediated clearance. The present study compared the sensitivity of polymerase chain reaction (PCR) to bacterial culture for detection of cerebral nocardial infection in this experimental system. Mice (n=4/time point) were administered N. asteroides by intravenous injection, and brain specimens were evaluated for Nocardia by PCR and culture at post-infection days 2, 7, 14 and 21. Nocardia was detected by PCR in all infected animals on post-infection days 2, 7, and 14, and in one of four mice on post-infection day 21; in contrast, the organism was detected by culture only on post-infection days 2 and 7. These findings suggest that PCR may be more sensitive than culture for the detection of low numbers of Nocardia in the brain.