The axonal localization of large Drosophila ankyrin2 protein Isoforms is essential for neuronal functionality
The axonal localization of large Drosophila ankyrin2 protein Isoforms is essential for neuronal functionality
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DOI：
10.1006/mcne.2002.1113
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发表时间：
2002-05-01
影响因子：
3.5
通讯作者：
Chandler, R
中科院分区：
文献类型：
--
作者：
Hortsch, M;Paisley, KL;Chandler, R
在极化细胞中，如神经元，锚定型蛋白是连接肌动蛋白-血影蛋白为基础的膜细胞骨架与质膜的主要分子。在果蝇中，第二个锚蛋白基因Dank 2仅在神经元细胞中表达。与其他生物体中的锚蛋白基因类似，Dank 2基因通过差异剪接产生几种锚蛋白同种型。在这里，我们报告说，在果蝇中，短Dank 2蛋白异构体仅限于神经元细胞体，并排除轴突，而长Dank 2异构体是专门定位于轴突。因此，长和短的Dank 2蛋白亚型定位于互补的神经元亚结构域，表明在体内的神经元皮质细胞骨架的组成是高度极化的。我们表明，一旦极化建立，它持续在果蝇发育的后期阶段。我们还提出了遗传证据，轴突Dank 2蛋白的缺乏是致命的。
In polarized cells, such as neurons, ankyrin-type proteins are the major molecules that link the actin-spectrinbased membrane cytoskeleton to the plasma membrane. In Drosophila the second ankyrin gene, Dank2, is exclusively expressed in neuronal cells. Similar to ankyrin genes in other organisms, the Dank2 gene generates several ankyrin protein isoforms by differential splicing. Here we report that in Drosophila, the short Dank2 protein isoform is restricted to neuronal cell bodies and is excluded from axons, whereas the long Dank2 isoforms are localized specifically to axons. Thus the long and short Dank2 protein isoforms are localized to complementary neuronal subdomains, demonstrating that in vivo the composition of the neuronal cortical cytoskeleton is highly polarized. We show that once polarization is established, it persists during later stages of Drosophila development. We also present genetic evidence that the absence of axonal Dank2 protein is lethal.