NMR characterization of RNA small molecule interactions
NMR characterization of RNA small molecule interactions
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DOI：
10.1016/j.ymeth.2019.05.015
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发表时间：
2019-09-01
期刊：
影响因子：
4.8
通讯作者：
Zhang, Qi
中科院分区：
文献类型：
--
作者：
Thompson, Rhese D.;Baisden, Jared T.;Zhang, Qi
天然存在的配体感应和与疾病相关的非编码 RNA 的令人兴奋的发现极大地促进了人们对理解 RNA-小分子相互作用的兴趣。核磁共振波谱是表征分子间相互作用的强大工具。在这篇综述中，我们描述了应用 NMR 波谱研究 RNA 和小分子之间相互作用的方案和方法。我们回顾了 RNA 样品制备方案、识别 RNA 结合小分子的方法、绘制 RNA-小分子相互作用、确定复杂结构和表征结合动力学的方法。我们希望这篇综述能够为简化 NMR 在研究 RNA-小分子相互作用中的应用提供指导，促进对 RNA 功能的基本机制理解和开发 RNA 靶向疗法的转化工作。
Exciting discoveries of naturally occurring ligand-sensing and disease-linked noncoding RNAs have promoted significant interests in understanding RNA-small molecule interactions. NMR spectroscopy is a powerful tool for characterizing intermolecular interactions. In this review, we describe protocols and approaches for applying NMR spectroscopy to investigate interactions between RNA and small molecules. We review protocols for RNA sample preparation, methods for identifying RNA-binding small molecules, approaches for mapping RNA-small molecule interactions, determining complex structures, and characterizing binding kinetics. We hope this review will provide a guideline to streamline NMR applications in studying RNA-small molecule interactions, facilitating both basic mechanistic under-standings of RNA functions and translational efforts in developing RNA-targeted therapeutics.