Use of rhizosphere microorganisms in the biodegradation of PLA and PET polymers in compost soil
Use of rhizosphere microorganisms in the biodegradation of PLA and PET polymers in compost soil
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DOI：
10.1016/j.ibiod.2018.03.017
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发表时间：
2018-05-01
影响因子：
4.8
通讯作者：
Dabrowska, Grazyna
中科院分区：
文献类型：
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作者：
Janczak, Katarzyna;Hrynkiewicz, Katarzyna;Dabrowska, Grazyna
这项工作研究了两种类型的聚合物的生物降解变化——生物可降解的（聚乳酸，PLA）和传统的（聚对苯二甲酸乙二醇酯，PET）——它们在工业上有类似的用途。本项目的主要目的是选择根际细菌和真菌作为植物生长促进微生物（PGPM）来加速土壤中PLA和PET的生物降解。以油菜（Brassica napus）、芒草（miscanthus x giganteus）和柳树（Salix viminalis）为材料，采用盆栽试验研究了聚合物在堆肥土壤中的生物降解。另外，通过接种两种细菌（磺化节杆菌、夹带沙雷菌）或两种真菌菌株（阴蒂菌、拉卡菌）刺激植物生长。选择实验中使用的菌株是根据它们的水解活性和在塑料上体外生长的能力。在细菌的情况下，在两种类型的聚合物上产生生物膜的能力被确定。在土壤中孵育6个月后，根据塑料表面结构和物理和化学性质的变化评估了塑料的生物降解变化。所分析的纸的生物降解速度最快的是S. plymuthica和L. laccata菌株的存在。接种土壤中PLA的存在降低了土壤pH值，增加了土壤的氧化还原电位，增加了土壤中真菌和细菌的丰度。试验证实了菌种对植物生物量的刺激作用。
This work examines biodegradational changes in two types of polymer-biodegradable (polylactide, PLA) and conventional (polyethylene terephthalate, PET)- which have similar uses in industry. The main objective of this project was to select rhizosphere bacteria and fungi that were plant growth promoting microorganisms (PGPM) to accelerate the biodegradation of PLA and PET in soil in which selected plant species were cultivated. A pot experiment was conducted to evaluate polymer biodegradation in compost soil in which three plant species were cultivated: rape (Brassica napus), miscanthus (Miscanthus x giganteus), and willow (Salix viminalis). Plant growth was additionally stimulated by inoculation with two bacterial (Arthrobacter sulfonivorans, Serratia plymuthica) or two fungal strains (Clitocybe sp., Laccaria laccata). The strains used in the experiment were selected for their hydrolytic activity and their ability to grow in vitro on plastics. In the case of the bacteria, the ability to create a biofilm on both types of studied polymers was identified. Biodegradation changes in the plastics were assessed based on changes in both their surface structure and physical and chemical properties after a 6-month incubation in the soil. Biodegradation of the analysed sheets was most rapid in the presence of the S. plymuthica and L. laccata strains. The presence of PLA in the inoculated soil lowered the soil pH and increased its redox potential as well as increased the abundance of fungi and bacteria in the soil. The experiment confirmed the stimulating effect of the S. plymuthica and L. laccata strains on plant biomass.