Synthesis and recognition of novel isonucleoside triphosphates by DNA polymerases
Synthesis and recognition of novel isonucleoside triphosphates by DNA polymerases
复制标题
DNA聚合酶合成和识别新型异核苷三磷酸
DOI：
10.1016/j.bmc.2007.02.003
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发表时间：
2007-04-15
影响因子：
3.5
通讯作者：
Zhang, Lihe
中科院分区：
文献类型：
--
作者：
Jiang, Caiwu;Li, Bingchao;Zhang, Lihe
近年来，异核苷因其化学和酶的稳定性以及潜在的抗癌和抗病毒活性而引起了人们的广泛关注。我们已经报道了一些具有显著抗癌活性的异核苷，并发现与异核苷结合的寡核苷酸可以提高酶的稳定性，抵抗磷酸二酯酶的降解。本文采用非放射性方法研究了Taq、Vent（exo(-)）、DeepVent（exo(-)）、9°Nm和Therminator DNA聚合酶对三磷酸异核苷1-6的识别。我们发现大多数三磷酸异核苷可以被多种DNA聚合酶识别并作为终止子。三磷酸异核苷2和6可以通过Vent（exo(-)）和DeepVent(exo(-)) DNA聚合酶作为底物加入到3'端引物中，依靠DNA模板延长链。(c) 2007 Elsevier Ltd.版权所有。
Isonucleosides have been attracting a lot of attention in recent years due to the chemical and enzymatic stability and potential anticancer and antiviral activities. We have reported some of the isonucleosides which exhibited significant anticancer activity and found that the oligonucleotide incorporated with isonucleoside could increase the enzymatic stability against the degradation by phosphodiesterase. In this paper, we investigated the recognition of the isonucleoside triphosphates 1-6 by Taq, Vent(exo(-)), DeepVent(exo(-)), 9 degrees Nm, and Therminator DNA polymerases by a non-radioactivity method. We found that most of the isonucleoside triphosphates can be recognized by various DNA polymerase and act as terminators. Isonucleoside triphosphates 2 and 6 can be incorporated as substrates into the primer at 3' terminus to lengthen the chain dependent on a DNA template by Vent(exo(-)) and DeepVent(exo(-)) DNA polymerases. (c) 2007 Elsevier Ltd. All rights reserved.