Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a truncated version of -glucosidase from Aspergillus niger
Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a truncated version of -glucosidase from Aspergillus niger
复制标题
通过来自黑曲霉的截短型 β-葡萄糖苷酶将抗坏血酸转葡萄糖基化为抗坏血酸 2-葡萄糖苷
DOI：
10.1111/jfbc.12432
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发表时间：
2017-12-01
影响因子：
4
通讯作者：
Yao, Quanhong
中科院分区：
文献类型：
--
作者：
Li, Zhenjun;Han, Hongjuan;Yao, Quanhong
A truncated version of -glucosidase gene (AglA) from Aspergillus niger, named as AglA-t in this study, was expressed in Pichia pastoris, and the enzyme was purified and characterized. Phylogenetic analysis demonstrated that AglA was most close to the -glucosidase from Aureobasidium pullulans. In our research, we found that AglA-t could promote the formation of 2-O--d-glucopyranosyl-l-ascorbic acid (AA-2G) under optimized conditions. The major product was detected by high performance liquid chromatography, and confirmed to be by LC-MS analysis. The optimum pH and temperature for the transglucosylation reaction were 6.0 degrees C and 40 degrees C, respectively. The yield of AA-2G was significantly enhanced by metal ions Fe3+, Zn2+, Mg2+, and Al3+, and organic solvent isopropanol.Practical applicationsHere, we described the biochemical properties of recombinant enzyme AglA-t which was used to promote AA-2G production. The high yield of AglA-t in Pichia pastoris makes it potentially useful for large-scale industrial application. Meanwhile, the chemically stable AA-2G, enzymatic product of AglA-t, could be applied to a wide range of fields due to its antioxidative ability, iron absorption, and carnitine synthesis. AA-2G plays an important role in maintaining skin elasticity and repairing damaged skin. Therefore, AA-2G could be used as a primary skin care ingredient in commercial cosmetics. In addition, AA-2G has great potential in human food and medical industries. In the animal husbandry and aquaculture fields, AA-2G is also widely added to forage as an important nutrition component. Taken together, the characterization of AglA-t in our research provides an alternative way for mass production of AA-2G which could be used for a variety of commercial applications.
