Testosterone application decreases the capacity for ACTH and corticosterone secretion in a rat model of the andropause
Testosterone application decreases the capacity for ACTH and corticosterone secretion in a rat model of the andropause
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DOI：
10.1016/j.acthis.2015.04.002
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发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Milosevic, Verica
中科院分区：
文献类型：
--
作者：
Ajdzanovic, Vladimir;Jaric, Ivana;Milosevic, Verica
男性衰老的最终阶段——男性更年期的特点是下丘脑-垂体-肾上腺轴活性增强和糖皮质激素频繁过量。与此同时，游离睾酮缺乏为老年男性提供了基线荷尔蒙环境。本研究的目的是（使用不同的微观和生化方法）说明睾酮应用对男性更年期大鼠模型促肾上腺皮质激素 (ACTH) 和皮质酮分泌能力的影响。中年 Wistar 大鼠分为假手术组 (SO; n = 8)、睾丸切除组 (Orx; n = 8) 和睾酮治疗睾丸切除组 (Orx +T; n = 8)。丙酸睾酮（5 毫克/公斤体重/天）持续三周，而 SO 组和 Orx 组仅接受媒介物。使用免疫组织化学、免疫荧光和组织化学程序对 ACTH 细胞和肾上腺皮质进行染色。通过免疫测定法测量睾酮、雌二醇、促肾上腺皮质激素和皮质酮的循环浓度以及肾上腺组织皮质酮水平。睾酮的应用导致性类固醇血清浓度增加（p < 0.05）。因此，与 Orx 组的相应参数相比，Orx+T 大鼠的 ACTH 细胞核体积增加 (p < 0.05) 34%，而 ACTH 细胞的体积密度及其相对荧光强度分别降低 (p < 0.05) 46% 和 21%。与 Orx 组相比，睾酮还诱导肾上腺皮质束状带血管舒张，并使 ACTH 浓度和肾上腺组织皮质酮水平分别降低 (p < 0.05) 38% 和 31%。总之，在雄性更年期大鼠模型中，施用睾酮导致 ACTH 和皮质酮分泌能力下降。 (C) 2015 爱思唯尔有限公司。版权所有。
The culminating phase of ageing in males-andropause is characterized by enhanced activity of the hypothalamic-pituitary-adrenal axis and frequent glucocorticoid excess. In parallel, free testosterone deficiency provides the baseline hormonal milieu for the ageing male. The aim of this study was to illustrate (using diverse microscopic and biochemical methodologies) the effects of testosterone application on the capacity for adrenocorticotropic hormone (ACTH) and corticosterone secretion in a rat model of the andropause. Middle-aged Wistar rats were divided into sham-operated (SO; n = 8), orchidectomized (Orx; n = 8) and testosterone treated orchidectomized (Orx +T; n = 8) groups. Testosterone propionate (5 mg/kg b.w./day) was administered for three weeks, while SO and Orx groups received the vehicle alone. ACTH cells and the adrenal cortex were stained using immuno-histochemical, immuno-fluorescent and histochemical procedures. Circulating concentrations of testosterone, estradiol, ACTH and corticosterone, as well as the adrenal tissue corticosterone levels were measured by immunoassays. Testosterone application led to increased (p < 0.05) serum concentrations of sex steroids. Consequently, in Orx+T rats the ACTH cell nuclei volume increased (p < 0.05) by 34%, while the volume density of ACTH cells and their relative intensity of fluorescence decreased (p < 0.05) by 46% and 21%, respectively, in comparison with the corresponding parameters in the Orx group. Testosterone also induced vasodilatation in the adrenocortical zona fasciculata, and decreased (p < 0.05) the ACTH concentrations and adrenal tissue corticosterone levels by 38% and 31%, respectively, compared to the Orx group. In conclusion, testosterone administration caused a decrease in the capacity for ACTH and corticosterone secretion in a rat model of the andropause. (C) 2015 Elsevier GmbH. All rights reserved.