The effects of Malassezia yeasts on cytokine production by human keratinocytes.
The effects of Malassezia yeasts on cytokine production by human keratinocytes.
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DOI：
10.1046/j.1523-1747.2001.01321.x
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发表时间：
2001-05
期刊：
影响因子：
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通讯作者：
Shinichi Watanabe;Rui Kano;Rui Kano;Hiroko Sato;Yuka Nakamura;Yuka Nakamura;Atsuhiko Hasegawa
中科院分区：
文献类型：
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作者：
Shinichi Watanabe;Rui Kano;Rui Kano;Hiroko Sato;Yuka Nakamura;Yuka Nakamura;Atsuhiko Hasegawa
马拉色菌酵母是皮肤微生物菌群的成员，可引起花斑糠疹，也与其他浅表皮肤病的发病机制有关；最重要的是脂溢性皮炎、毛囊炎和特应性皮炎。然而，酵母引起这些皮肤病的机制尚不清楚，真菌与角质形成细胞之间的相互作用尚未得到研究，特别是真菌对人角质形成细胞产生细胞因子的影响。最近，根据分子数据，马拉色菌属已扩展到7种。本研究通过测定马拉色菌对人角质形成细胞产生细胞因子（白细胞介素1 β、6、8、单核细胞趋化蛋白-1、肿瘤坏死因子- α）的影响，探讨不同马拉色菌的致病性是否存在差异，阐明马拉色菌引起具有不同临床病理表现的皮肤病的机制。在马拉色酵母（除M. furfur外）共培养1至24小时后，上清液中白细胞介素6、8和肿瘤坏死因子α的变化水平升高，但单核细胞趋化蛋白-1未检测到。此外，仅用马拉色菌上清收获角质形成细胞1-24小时后，上清液中的细胞因子水平无法检测到。这些结果表明，马拉色菌可以刺激人角质形成细胞产生细胞因子，细胞因子的产生需要马拉色菌的存在，不同马拉色菌诱导人角质形成细胞产生细胞因子的能力存在差异。这些差异可能反映了马拉色菌相关皮肤病的不同炎症反应，导致不同的临床和病理表现。
Yeasts of Malassezia, members of the microbiologic flora of the skin, cause pityriasis versicolor and have also been implicated in the pathogenesis of other superficial dermatoses; the most important ones are seborrheic dermatitis, folliculitis, and atopic dermatitis. The mechanisms by which the yeasts cause these dermatoseş however, are not yet clear, and there have been no studies on the interaction between fungi and keratinocytes, especially the effects of fungi on the production of cytokines by human keratinocytes. Recently, the genus Malassezia has been expanded to seven species based on molecular data. In this study, we estimated the effects of Malassezia yeasts on cytokine (interleukins 1beta, 6, and 8, monocyte chemotactic protein-1, and tumor necrosis factor-alpha) production by human keratinocytes in order to examine whether the pathogenicity of the respective Malassezia yeasts is different from each other and to elucidate the mechanism by which Malassezia yeasts cause the dermatoses with different clinical and pathologic manifestations. Variable levels of interleukin 6 and 8, and tumor necrosis factor-alpha in the supernatants in response to Malassezia yeasts (except M. furfur) increased from 1 to 24 h co-culture, but the monocyte chemotactic protein-1 was undetectable. Furthermore, cytokine levels in the supernatants were undetectable 1-24 h after the keratinocytes were harvested with only supernatants of Malassezia. These results indicate that Malassezia stimulates cytokine production by keratinocytes, the cytokine production needs the presence of Malassezia, and there are differences in ability to induce cytokine production by human keratinocytes among Malassezia yeasts. These differences may reflect the different inflammatory responses in Malassezia-associated dermatoses, resulting in different clinical and pathologic manifestations.