INTERACTIONS OF SOME ACCEPTORS WITH SUPEROXIDE ANION RADICALS FORMED BY NADPH-SPECIFIC FLAVOPROTEIN IN RAT-LIVER MICROSOMAL FRACTIONS
INTERACTIONS OF SOME ACCEPTORS WITH SUPEROXIDE ANION RADICALS FORMED BY NADPH-SPECIFIC FLAVOPROTEIN IN RAT-LIVER MICROSOMAL FRACTIONS
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DOI：
10.1042/bj1540307
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发表时间：
1976-01-01
影响因子：
4.1
通讯作者：
LYAKHOVICH, VV
中科院分区：
文献类型：
--
作者：
MISHIN, V;POKROVSKY, A;LYAKHOVICH, VV
在大鼠肝微粒体部分中，肾上腺素的氧化受到超氧阴离子自由基 (O2-) 的影响，而细胞色素 c、2,6-二氯苯酚-靛酚和铁氰化物仅直接接受来自 NADPH 特异性黄素蛋白的电子。硝基蓝四唑被 O2- 和直接接受电子还原。 pH 值升高和甲萘醌的添加将硝基蓝四唑的还原转向 O2 依赖性途径。根据肾上腺素和硝基蓝四唑与 NADPH 特异性黄素蛋白相互作用的动力学常数值，确定大鼠肝微粒体部分中 O2- 的生成率。
In rat liver microsomal fractions oxidation of adrenaline was effected by superoxide anion radicals (O2-), whereas cytochrome c, 2,6-dichlorophenol-indophenol and ferricyanide accepted electrons from NADPH-specific flavoprotein only directly. Nitro Blue Tetrazolium was reduced both by O2-and by the direct acceptance of electrons. Elevation of pH and addition of menadione shift the Nitro Blue Tetrazolium reduction towards the O2--dependent pathway. From the values of the kinetic constants for interaction of adrenaline and Nitro Blue Tetrazolium with NADPH-specific flavoprotein, the rates of generation of O2-in rat liver microsomal fraction were determined.