Somatotopy of the Neurons Innervating the Cricothyroid, Posterior Cricoarytenoid, and Thyroarytenoid Muscles of the Rat's Larynx
Somatotopy of the Neurons Innervating the Cricothyroid, Posterior Cricoarytenoid, and Thyroarytenoid Muscles of the Rat's Larynx
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DOI：
10.1002/ar.22643
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发表时间：
2013-03-01
影响因子：
2
通讯作者：
Valderrama-Canales, Francisco J.
中科院分区：
文献类型：
--
作者：
Hernandez-Morato, Ignacio;Pascual-Font, Aran;Valderrama-Canales, Francisco J.
支配喉内肌的神经元位于疑核内，但每块肌肉的神经元的精确分布仍然是一个有争议的问题。本研究的目的是精细确定大鼠喉内肌环甲肌、环杓后肌和甲杓肌神经元的位置和数量。该研究在总共 28 只 Sprague Dawley 大鼠中进行。霍乱毒素的 B 亚基被用作逆行示踪剂，以确定肌内注射后这些内在喉肌的神经元在疑核内的位置。在动物样本中，在同侧的疑核中发现了标记的神经元，这些神经元被分组为离散的群体，在动物样本中具有可重复的头尾和背腹位置。支配环甲肌的神经元位于三个群体中最靠近嘴侧的位置，支配环杓状肌后部的神经元位于尾侧，尽管这两个群体之间存在头尾重叠区域。最尾侧的是支配甲杓肌的神经元，与后环杓肌有不同程度的重叠。环甲腺标记神经元的平均数量 (+/-SD) 为 41 +/- 9，后环杓状细胞为 39 +/- 10，甲状杓状细胞为 33 +/- 12。 Anat Rec，296：470-479，2013。(C)2013 Wiley 期刊公司。
Neurons innervating the intrinsic muscles of the larynx are located within the nucleus ambiguus but the precise distribution of the neurons for each muscle is still a matter for debate. The purpose of this study was to finely determine the position and the number of the neurons innervating the intrinsic laryngeal muscles cricothyroid, posterior cricoarytenoid, and thyroarytenoid in the rat. The study was carried out in a total of 28 Sprague Dawley rats. The B subunit of the cholera toxin was employed as a retrograde tracer to determine the locations, within the nucleus ambiguus, of the neurons of these intrinsic laryngeal muscles following intramuscular injection. The labelled neurons were found ipsilaterally in the nucleus ambiguus grouped in discrete populations with reproducible rostrocaudal and dorsoventral locations among the sample of animals. Neurons innervating the cricothyroid muscle were located the most rostral of the three populations, neurons innervating the posterior cricoarytenoid were found more caudal, though there was a region of rostrocaudal overlap between these two populations. The most caudal were the neurons innervating the thyroarytenoid muscle, presenting a variable degree of overlap with the posterior cricoarytenoid muscle. The mean number (+/-SD) of labelled neurons was found to be 41 +/- 9 for the cricothyroid, 39 +/- 10 for the posterior cricoarytenoid and 33 +/- 12 for the thyroarytenoid. Anat Rec, 296: 470-479, 2013. (C) 2013 Wiley Periodicals, Inc.