Identification of envelope protein residues required for the expanded host range of 10A1 murine leukemia virus
Identification of envelope protein residues required for the expanded host range of 10A1 murine leukemia virus
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DOI：
10.1128/jvi.71.11.8103-8108.1997
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发表时间：
1997-11-01
影响因子：
5.4
通讯作者：
Anderson, WF
中科院分区：
文献类型：
--
作者：
Han, JY;Cannon, PM;Anderson, WF
10A 1鼠白血病病毒（MuLV）是一种重组C型逆转录病毒，分离自嗜中性MuLV（A-MuLV）感染的小鼠。10A 1和A-MuLV在其包膜蛋白中具有91%的氨基酸同一性，但显示不同的宿主范围。例如，CHO-K1细胞对A-MuLV具有抗性，但对10A 1感染敏感。我们现在已经确定，携带在位置71（A71 G）、74（Q74 K）和139（V139 M）处含有10A 1衍生残基的改变的A-MuLV包膜蛋白的逆转录病毒载体以与用10A 1包膜载体实现的那些类似的效率转染CHO-K1细胞。具有这三个10A 1残基的A-MuLV包膜逆转录病毒载体也能够抑制A-MuLV感染的NIH 3 T3细胞。这一观察结果与携带这种改变的A-MuLV包膜蛋白的载体识别NIH 3 T3细胞上存在的10A 1特异性受体的能力一致，并支持10A 1包膜SU蛋白的71、74和139位残基解释10A 1的宿主范围扩大的可能性。
The 10A1 murine leukemia virus (MuLV) is a recombinant type C retrovirus isolated from a mouse infected with amphotropic MuLV (A-MuLV). 10A1 and A-MuLV have 91% amino acid identity in their envelope proteins yet display different host ranges. For example, CHO-K1 cells are resistant to A-MuLV but susceptible to infection by 10A1. We have now determined that retroviral vectors bearing altered A-MuLV envelope proteins containing 10A1-derived residues at positions 71 (A71G), 74 (Q74K), and 139 (V139M) transduce CHO-K1 cells at efficiencies similar to those achieved with 10A1 enveloped vectors. A-MuLV enveloped retroviral vectors with these three 10A1 residues were also able to transduce A-MuLV-infected NIH 3T3 cells. This observation is consistent with the ability of vectors bearing this altered A-MuLV envelope protein to recognize the 10A1-specific receptor present on NIH 3T3 cells and supports the possibility that residues at positions 71, 74, and 139 of the 10A1 envelope SU protein account for the expanded host range of 10A1.