Surface-segregating zwitterionic copolymers to control poly(dimethylsiloxane) surface chemistry.
Surface-segregating zwitterionic copolymers to control poly(dimethylsiloxane) surface chemistry.
复制标题
用于控制聚（二甲基硅氧烷）表面化学的表面分离两性离子共聚物。
DOI：
10.1039/d3tb02164e
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Asatekin,Ayse
中科院分区：
文献类型：
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作者：
Gokaltun,AAslihan;Mazzaferro,Luca;Yarmush,MartinL;Usta,OBerk;Asatekin,Ayse
微流控装置在生物医学中的应用正在快速增长，例如器官芯片和分离。聚二甲基硅氧烷（PDMS）是最受欢迎的微流体材料，因为它能够复制到纳米级的功能，灵活性，透气性和低成本。然而，PDMS固有的疏水性导致大分子和小分子在器件表面上的吸附。这限制了它在“芯片上器官”和其他应用中的使用。目前改善PDMS表面亲水性和耐污染性的技术涉及额外的加工步骤，或者不会产生长期保持亲水性的表面。这项工作描述了一种新的，简单，快速，可扩展的方法，用于改善表面亲水性和防止蛋白质和小分子的非特异性吸附PDMS通过使用的表面分离的两性离子共聚物作为添加剂，在制造过程中混合。这些高度支化的共聚物自发地分离到表面，并在与水溶液接触时重新排列以抵抗非特异性吸附。我们报告说，在PDMS中混合微量（0.025重量%）的两性离子共聚物大大降低了蛋白质（白蛋白和溶菌酶）和小分子（维生素B12和活性红）的疏水性和非特异性吸附。与这些两性离子共聚物共混的PDMS保持其机械和物理性能至少六个月。此外，这种方法与现有的PDMS装置制造方案完全兼容，而无需额外的处理步骤，因此提供了一种低成本和用户友好的方法来制造可靠的生物微流体。
The use of microfluidic devices in biomedicine is growing rapidly in applications such as organs-on-chip and separations. Polydimethylsiloxane (PDMS) is the most popular material for microfluidics due to its ability to replicate features down to the nanoscale, flexibility, gas permeability, and low cost. However, the inherent hydrophobicity of PDMS leads to the adsorption of macromolecules and small molecules on device surfaces. This curtails its use in “organs-on-chip” and other applications. Current technologies to improve PDMS surface hydrophilicity and fouling resistance involve added processing steps or do not create surfaces that remain hydrophilic for long periods. This work describes a novel, simple, fast, and scalable method for improving surface hydrophilicity and preventing the nonspecific adsorption of proteins and small molecules on PDMS through the use of a surface-segregating zwitterionic copolymer as an additive that is blended in during manufacture. These highly branched copolymers spontaneously segregate to surfaces and rearrange in contact with aqueous solutions to resist nonspecific adsorption. We report that mixing a minute amount (0.025 wt%) of the zwitterionic copolymer in PDMS considerably reduces hydrophobicity and nonspecific adsorption of proteins (albumin and lysozyme) and small molecules (vitamin B12 and reactive red). PDMS blended with these zwitterionic copolymers retains its mechanical and physical properties for at least six months. Moreover, this approach is fully compatible with existing PDMS device manufacture protocols without additional processing steps and thus provides a low-cost and user-friendly approach to fabricating reliable biomicrofluidics.