Model of Evoked Rabbit Phonation
Model of Evoked Rabbit Phonation
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DOI：
10.1177/000348940911800109
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发表时间：
2009-01-01
影响因子：
1.4
通讯作者：
Rousseau, Bernard
中科院分区：
文献类型：
--
作者：
Ge, Ping Jiang;French, Lesley C.;Rousseau, Bernard
目的：我们描述了一种方法，用于引发发声在体内兔制备使用低频，双极脉冲刺激的环甲肌与气流传递到glottis.Methods：10新西兰白色种兔体重3至5公斤，在这项研究中使用。双侧分离环甲肌，并将单独的阳极-阴极钩丝电极对插入每块肌肉。A草S-88刺激器和2个恒流PSIU 6隔离单元被用来提供双极方波脉冲到每个环甲肌，通过一个带套囊的气管内tube.Results气流传递到声门：发声诱发与50 Hz，4 mA刺激1毫秒脉冲序列传递到每个环甲肌。脉冲串持续2秒，并在180分钟的时间段内每5秒重复一次。以143 cm（3）/s的速度输送气流，产生测量为71 ~ 85 dB声压级的发声。结论：通过使用双极刺激环甲肌并将气流输送到声门，诱发发声在兔中是可行的。所描述的在体兔制备可以为诱发发声的研究提供有用的小动物选择。从观察到的内收的水平和一致性，我们假设，电流传播到下面的内收肌和神经导致神经通路参与超出离散激活的环甲肌，提供足够的近似发声的声带。
Objectives: We describe a method for eliciting phonation in an in vivo rabbit preparation using low-frequency, bipolar pulsed stimulation of the cricothyroid muscles with airflow delivered to the glottis.Methods: Ten New Zealand White breeder rabbits weighing 3 to 5 kg were used in this study. The cricothyroid muscles were isolated bilaterally, and separate pairs of anode-cathode hooked-wire electrodes were inserted into each Muscle. A Grass S-88 stimulator and 2 constant-current PSIU6 isolation units were used to deliver bipolar square wave pulses to each cricothyroid muscle, with airflow delivered to the glottis through a Cuffed endotracheal tube.Results: Phonation was evoked with a 50-Hz, 4-mA stimulus train of 1-ms pulses delivered to each cricothyroid muscle. The pulse trains were oil for 2 seconds and were repeated every 5 seconds over a period Of 180 Minutes. Airflow was delivered at 143 cm(3)/s, producing phonation measuring 71 to 85 dB Sound pressure level.Conclusions: Evoked phonation is feasible in rabbits by use of bipolar stimulation of the cricothyroid muscles with airflow delivered to the glottis. The in vivo rabbit preparation described may provide a useful small animal option for studies of evoked phonation. From the level and consistency of the adduction observed, we hypothesize that current spreading to the underlying adductor muscles and nerves resulted in neural pathway involvement beyond discrete activation of the cricothyroid muscle, providing sufficient approximation of the vocal folds for phonation.