Advances in structural and functional analysis of membrane proteins by electron crystallography.
Advances in structural and functional analysis of membrane proteins by electron crystallography.
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电子晶体学膜蛋白结构和功能分析的进展。
DOI：
10.1016/j.str.2011.09.001
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Gonen,Tamir
中科院分区：
文献类型：
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作者：
Wisedchaisri,Goragot;Reichow,SteveL;Gonen,Tamir
电子晶体学是研究膜蛋白在脂质环境中的结构和功能的有力技术。当获得有序的二维晶体时，可以确定蛋白质和脂质的结构，并分析脂质-蛋白质相互作用。质子和离子电荷可以通过电子晶体学可视化，并且可以捕获感兴趣的蛋白质用于各种生理学不同状态的结构分析。这篇综述强调了电子晶体学的优势和势头，建立在自动化和高通量的工具和方法的开发膜蛋白的结构和功能分析的电子晶体学。
Electron crystallography is a powerful technique for the study of membrane protein structure and function in the lipid environment. When well-ordered two-dimensional crystals are obtained the structure of both protein and lipid can be determined and lipid-protein interactions analyzed. Protons and ionic charges can be visualized by electron crystallography and the protein of interest can be captured for structural analysis in a variety of physiologically distinct states. This review highlights the strengths of electron crystallography and the momentum that is building up in automation and the development of high throughput tools and methods for structural and functional analysis of membrane proteins by electron crystallography.