INFECTION OF LEUKOCYTE CELL-CULTURES DERIVED FROM DIFFERENT SPECIES WITH PIG CIRCOVIRUS
INFECTION OF LEUKOCYTE CELL-CULTURES DERIVED FROM DIFFERENT SPECIES WITH PIG CIRCOVIRUS
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DOI：
10.1016/0378-1135(94)90107-4
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发表时间：
1994-08-01
影响因子：
3.3
通讯作者：
ADAIR, BM
中科院分区：
文献类型：
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作者：
ALLAN, GM;MCNEILLY, F;ADAIR, BM
从猪骨髓、外周血、肺洗液、胸腺和淋巴结制备白细胞培养物。还从绵羊、牛和人的外周血制备细胞培养物。接种猪圆环病毒（PCV）后，对所有这些培养物进行免疫荧光（IF）染色，检测到来自猪的所有细胞培养物和来自牛的细胞培养物中的病毒复制。通过证明感染性病毒的产生，证实了猪白细胞细胞培养物中的病毒复制。使用细胞膜标记物特异性单克隆抗体对PCV感染细胞进行双重免疫染色，表明感染仅限于单核细胞/巨噬细胞类型。在感染细胞培养物的T或B细胞中未检测到PCV抗原。
Cultures of leucocyte cells were prepared from pig bone marrow, peripheral blood, lung washings, thymus and lymph nodes. Cell cultures were also prepared from peripheral blood from sheep, cattle and a human. Immunofluorescent (IF) staining of all these cultures, following inoculation with pig circovirus (PCV), detected virus replication in all the cell cultures derived from pigs and in the cell cultures derived from cattle. Virus replication in pig leucocyte cell cultures was confirmed by demonstrating the production of infectious virus. Double immunostaining of PCV infected cells using monoclonal antibodies specific for cell membrane markers indicated infection was confined to monocyte/macrophage cell types. No PCV antigen was detected in T or B cells in infected cell cultures.