Nogo-A expression in mouse central nervous system neurons
Nogo-A expression in mouse central nervous system neurons
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DOI：
10.1016/s0304-3940(02)00528-1
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发表时间：
2002-08-16
影响因子：
2.5
通讯作者：
Tang, BL
中科院分区：
文献类型：
--
作者：
Liu, HP;Ng, CEL;Tang, BL
最长的中枢神经系统（CNS）特异性亚型的主要髓鞘相关的神经突起生长抑制剂，Nogo-A，以前已被证明主要在少突胶质细胞中表达。使用针对包含Nogo-A的氨基酸223-399的重组融合蛋白产生的抗体，我们在本报告中显示Nogo-A也在一组不同的CNS神经元的细胞体中表达。该抗体可检测大鼠脑裂解物和神经母细胞瘤细胞裂解物中220 kDa的单一蛋白条带。免疫荧光分析显示Nogo-A主要存在于神经母细胞瘤细胞系SH-SY 5 Y和NIE-115的内质网中。在小鼠CNS中，Nogo-A可以在除少突胶质细胞之外的神经元细胞体的特定亚群中发现，但不存在胶质细胞相关蛋白阳性星形胶质细胞或MAC-1阳性小胶质细胞。我们的研究结果提供了一个决定性的证据Nogo-A在中枢神经系统神经元中的表达，这表明Nogo-A可能有不同的内源性作用，在神经元以外的其他已知的能力，以抑制神经突起的生长。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
The longest central nervous system (CNS) specific isoform of the major myelin-associated inhibitor of neurite growth, Nogo-A, has previously been shown to be expressed largely in oligodendrocytes. Using an antibody raised against a recombinant fusion protein comprising amino acids 223-399 of Nogo-A, we show in this report that Nogo-A is also expressed in the cell body of a distinct set of CNS neurons. The antibody detects a single protein band of 220 kDa in rat brain lysate and neuroblastoma cell lysates. Immunofluorescent analyses reveal that Nogo-A is found largely in the endoplasmic reticulum of neuroblastoma cell lines SH-SY5Y and NIE-115. In the mouse CNS, Nogo-A can be found in specific subsets of neuronal cell bodies in addition to oligodendrocytes, but not glial fibrillary associated protein positive astrocytes or MAC-1 positive microglia. Our results provide a conclusive demonstration of the expression of Nogo-A in CNS neurons, which suggests that Nogo-A may have distinct endogenous roles in neurons other than its known ability to inhibit neurite growth. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.