Collection of villous tissue under ultrasound guidance to improve the cytogenetic study of early pregnancy failure
Collection of villous tissue under ultrasound guidance to improve the cytogenetic study of early pregnancy failure
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DOI：
10.1093/humrep/17.2.452
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发表时间：
2002-02-01
影响因子：
6.1
通讯作者：
Jauniaux, E
中科院分区：
文献类型：
--
作者：
Greenwold, N;Jauniaux, E
背景资料：自然流产的细胞遗传学研究一直受到手术清除残留妊娠产物后细胞培养获得的核型成功率低的限制。本研究的目的是评估手术时绒毛组织收集方法的改进对细胞培养核型成功率的影响。研究方法：在手术开始时，通过超声成像引导小活检钳进入胎盘，前瞻性地从170例自发性流产的队列中获得绒毛样本。这是一个回顾性系列的1191自然流产，培养在同一实验室，传统的收集样本，从抽吸受体手术后。结果如下：在前瞻性系列中，6例（3.5%）原始样本被实验室分类为“仅蜕膜”，而在回顾性系列中为162例（13.6%）。前瞻性研究的核型成功率为94.5%，而回顾性研究的核型成功率为83.7%。染色体核型分析结果显示，前瞻性组和回顾性组的染色体异常率分别为65.8%和64%，两组的分布相似。结论：我们的数据表明，可以从流产手术时收集的干净绒毛材料中获得核型。因此，这是不合理的受试者经腹绒毛膜绒毛取样，以达到高核型成功率。
Background: The cytogenetic study of spontaneous miscarriage has been limited by poor karyotype success rates obtained from cell culture after surgical evacuation of retained products of conception. The aim of this study was to assess the effect of improving the method of collection of villous tissues at the time of surgery on the karyotype success rate of cell culture. Methods: Villous samples were obtained prospectively from a cohort of 170 spontaneous miscarriages at the beginning of the surgical procedure using small biopsy forceps guided into the placenta by ultrasound imaging. This was compared with a retrospective series of 1191 spontaneous miscarriages, cultured in the same laboratory, following conventional collection of the sample from the aspiration recipient after surgery. Results: In the prospective series, six (3.5%) of the original samples were classified by the laboratory as 'decidua only' as compared with 162 (13.6%) in the retrospective series. The karyotype success rate was 94.5% in the prospective series compared with 83.7% in the retrospective series. The karyotype results revealed a chromosome abnormality rate of 65.8% in the prospective group and 64% in the retrospective group with a similar distribution in both groups. Conclusions: Our data show that a karyotype can be obtained from clean villous material collected at the time of surgical evacuation of miscarriage. Thus, it is not justified to subject women to transabdominal chorionic villus sampling to achieve a high karyotype success rate.