EVIDENCE FOR AXONAL LOSS IN REGIONS OCCUPIED BY SENILE PLAQUES IN ALZHEIMER CORTEX
EVIDENCE FOR AXONAL LOSS IN REGIONS OCCUPIED BY SENILE PLAQUES IN ALZHEIMER CORTEX
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DOI：
10.1016/0306-4522(91)90034-l
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发表时间：
1991-01-01
期刊：
影响因子：
3.3
通讯作者：
BIRD, ED
中科院分区：
文献类型：
--
作者：
BENES, FM;FAROL, PA;BIRD, ED
所描述的研究试图确定轴突和老年斑之间存在什么关系（如果有的话），老年斑是阿尔茨海默病的标志性组织病理学特征。对早期和晚期阿尔茨海默额叶皮质组织进行双重染色，以检查用抗200，000 mol.轴突细胞骨架的wt神经丝亚基（NFP-200）和硫磺素-S（一种染色斑块的荧光染料）。拍摄了斑块的系列显微照片，并对轴突和斑块轮廓进行了三维重建。对计算机处理图像的分析显示，斑块内的轴突比距离一个或两个斑块的区域少。当观察到轴突穿过斑块时，经常出现正常形态的肿胀和破坏。轴突计数内和周围的斑块的统计分析显示，轴突密度的梯度，与数量增加发生在进步的距离从斑块。在疾病的早期和晚期也可以看到类似的模式。这项研究的结果表明，轴突细胞骨架的破坏可能发生在斑块所占据的区域内。
The studies described have sought to determine what, if any, relationship exists between axons and the senile plaque, a hallmark histopathological feature of Alzheimer's disease. A double stain was performed on both early and late Alzheimer frontal cortex tissues in order to examine the interaction between axons stained with antibodies against the 200,000 mol. wt neurofilament subunit (NFP-200) of the axon cytoskeleton and Thioflavin-S, a fluorescent dye that stains plaques. Serial photomicrographs of plaques were taken and axon and plaque profiles were three-dimensionally reconstructed. Analysis of computer-processed images revealed that there were fewer axons within plaques than in regions lying one and two plaque distances away. When axons were observed passing through plaques, swelling and disruption of normal morphology was frequently present. Statistical analyses of axon counts within and around placques showed a gradient of axon density, with increased numbers occurring at progressive distances from the placque. Similar patterns were seen for early and late stages of the disease.The results of this study indicate that disruption of the axonal cytoskeleton may occur within the regions occupied by plaques.