Genes expressed in sugarcane maturing internodal tissue
Genes expressed in sugarcane maturing internodal tissue
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DOI：
10.1007/s00299-002-0444-1
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发表时间：
2002-05-01
影响因子：
6.2
通讯作者：
Botha, FC
中科院分区：
文献类型：
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作者：
Carson, DL;Botha, FC
为了探究甘蔗茎杆成熟过程中的基因表达情况，我们对来自成熟茎杆的总cDNA文库和扣除文库的随机克隆进行了部分序列分析。数据库比对显示，在分析的337个cDNA序列中，167个与蛋白质数据库中的基因产物具有序列同源性，而111个仅与未表征的植物表达序列标签（ESTs）匹配。其余的cDNA在数据库中没有匹配项，可能代表新基因。大多数ESTs对应于与一般细胞代谢相关的各种基因。与各种应激反应基因同源的ESTs也有很好的体现。对扣除文库中ESTs的分析鉴定出了可能在茎杆成熟过程中优先表达的基因。这项研究为甘蔗蔗糖积累组织中的功能基因分析提供了一个框架。
To explore gene expression during sugarcane culm maturation, we performed a partial sequence analysis of random clones from maturing culm total and subtracted cDNA libraries. Database comparisons revealed that of the 337 cDNA sequences analysed, 167 showed sequence homology to gene products in the protein databases, while 111 matched uncharacterised plant expressed sequence tags (ESTs) only. The remaining cDNAs showed no database match and could represent novel genes. The majority of ESTs corresponded to a variety of genes associated with general cellular metabolism. ESTs homologous to various stress response genes were also well represented. Analysis of ESTs from the subtracted library identified genes that may be preferentially expressed during culm maturation. This research has provided a framework for functional gene analysis in sugarcane sucrose-accumulating tissues.