RESTRICTION OF HERPES SIMPLEX VIRUS BY MACROPHAGES
RESTRICTION OF HERPES SIMPLEX VIRUS BY MACROPHAGES
复制标题
巨噬细胞对单纯疱疹病毒的限制
DOI：
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发表时间：
1971
影响因子：
15.3
通讯作者：
M. L. Cook
中科院分区：
文献类型：
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作者：
J. G. Stevens;M. L. Cook
巨噬细胞限制单纯疱疹病毒的复制，可以防止小鼠疱疹疾病的发展。为了确定这种限制的性质，对感染巨噬细胞中病毒特异性大分子合成进行了分析。结果如下：所有细胞均被杀死，但感染被认为是失败的，因为感染后25小时，巨噬细胞培养物中的感染性病毒水平稳步下降到无法检测的水平。这种限制似乎是巨噬细胞所特有的；这种病毒在其他小鼠细胞中有效地复制。从感染培养物中提取具有单纯疱疹病毒DNA密度特征的DNA，感染后6小时巨噬细胞合成DNA的比例从不到1%增加到大于50%。聚丙烯酰胺凝胶电泳研究表明，主要的病毒特异性蛋白在巨噬细胞培养中被诱导。此外，所有表现出疱疹病毒感染特征的细胞病变的细胞也含有可以通过荧光抗体技术检测到的病毒抗原，并且在感染后15小时，大多数细胞含有缺乏中央致密核的新生衣壳。有人认为，DNA代谢的错误可能是限制的主要原因。
Macrophages restrict herpes simplex virus replication and can prevent the development of herpetic disease in mice. In an attempt to define the nature of this restriction, an analysis of virus-specified macromolecular syntheses in infected macrophages was undertaken. The significant results were the following: All cells were killed, but the infection was considered to be abortive since the level of infectious virus in macrophage cultures dropped steadily to a level beyond detection by 25 hr after infection. This restriction appeared to be specific for macrophages; the virus replicated efficiently in other mouse cells. DNA with a density characteristic for herpes simplex virus DNA was extracted from infected cultures, and the proportion of macrophages synthesizing DNA increased from less than 1% to greater than 50% by 6 hr after infection. Studies employing polyacrylamide gel electrophoresis indicated that the major viral-specific proteins were induced in macrophage cultures. In addition, all cells showing cytopathic changes characteristic of herpes virus infection also contained viral antigens which could be detected by fluorescent antibody techniques and, by 15 hr after infection, most contained nascent capsids lacking central dense cores. It is suggested that an error in DNA metabolism may be the primary cause of restriction.