DsCaf1 is involved in environmental stress response of Dunaliella salina
DsCaf1 is involved in environmental stress response of Dunaliella salina
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DsCaf1 参与杜氏盐藻的环境应激反应
DOI：
10.1016/j.ijbiomac.2015.10.015
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发表时间：
2016-01-01
影响因子：
8.2
通讯作者：
Yan, Yong-Bin
中科院分区：
文献类型：
--
作者：
Chen, Xiang-Jun;Zhang, Xin-Hang;Yan, Yong-Bin
mRNA命运的调节可以迅速而特异地改变基因表达谱。脱腺苷酸化是多聚（A）尾被去腺苷酸酶降解的过程。通过调节mRNA周转，去腺苷酸酶参与各种重要的细胞过程，包括应激反应。然而，目前还不清楚deadenylases是否在极端微生物的适应/耐受性中发挥作用。本研究克隆了杜氏盐藻（Dunaliella salina，dscaf 1）的脱腺苷酸酶帽基因。杜氏盐藻是一种单细胞绿色盐藻，具有特殊的耐盐性。硅分析表明，与嗜温的莱茵衣藻相比，dscaf 1启动子含有更多的响应非生物胁迫的元件。Dscaf 1在高盐条件下有极高的表达水平。当D. salina细胞受到应激休克，观察到dscaf 1表达的两阶段反应。高渗、热或紫外线处理后dscaf 1 mRNA水平迅速增加2-4倍，随后增加10倍，而低渗或冷休克处理后dscaf 1 mRNA水平迅速增加约3倍，随后突然下降。dscaf 1表达模式的差异表明DsCaf 1是一种应激反应性去腺苷酶，能够调节特定类型应激的特定mRNA组的命运。(c)2015爱思唯尔B. V.保留所有权利。
Regulation of mRNA fates can alter the gene-expression profile promptly and specifically. Deadenylation is a process of which the poly(A) tail is degraded by deadenylases. By regulating mRNA turnover, deadenylases are involved in various vital cellular processes including stress responses. However, it is unclear whether deadenylases play a role in the adaption/tolerance of extremophiles. In this research, we cloned the deadenylase cap from Dunaliella salina (dscaf1), a unicellular green alga with exceptional halotolerance. In silicon analysis indicated that compared with the mesophilic alga Chlamydomonas reinhardtii call, dscaf1 promoter contained more elements responsive to abiotic stresses. Dscaf1 had an extremely high expression level under hypersaline conditions. When the D. salina cells were subject to stress shock, a two-stage response was observed for dscaf1 expression. The mRNA level of dscaf1 had an immediate 2-4 fold increase and followed by an 10 fold increase after hyperosmotic, heat or UV treatment, while had an about 3 fold increase quickly followed by an abrupt decrease after hypoosmotic or cold shock. The dissimilarity in dscaf1 expression patterns suggested that DsCaf1 is a stress-responsive deadenylase with the ability to regulate fates of a specific group of mRNAs for a certain type of stress. (c) 2015 Elsevier B.V. All rights reserved.