Bone-protecting effect of safflower seeds in ovariectomized rats
Bone-protecting effect of safflower seeds in ovariectomized rats
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DOI：
10.1007/s00223-001-1080-4
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发表时间：
2002-07-01
影响因子：
4.2
通讯作者：
Lee, WJ
中科院分区：
文献类型：
--
作者：
Kim, HJ;Bae, YC;Lee, WJ
红花（Carthamus tinctorius L.）种子在韩国长期被临床用于促进骨形成和预防骨质疏松症。然而，其有益效果尚未得到科学评估。因此，在本研究中，我们研究了富含植物雌激素的红花种子是否能减少去卵巢大鼠的骨质流失。雌性Sprague-Dawley大鼠进行双侧卵巢切除术或假手术。手术后一周，切除卵巢的大鼠喂食含有脱脂红花籽的饮食或注射17 -雌二醇（E-2） 4周。正如预期的那样，卵巢切除术导致胫骨近端骨小梁骨量急剧减少，骨髓脂肪沉积增加，子宫萎缩。通过扫描电镜和组织形态计量学检查，E-2治疗几乎完全防止了骨质流失和骨髓肥胖。红花籽部分预防卵巢切除术引起的骨质流失，并略微减少骨髓脂肪。与E-2相比，红花种子的子宫营养作用非常弱。为了阐明其潜在的机制，我们还在体外评估了从红花种子中提取的多酚类化合物对成骨样细胞增殖的影响。混合多酚类化合物以剂量依赖性的方式（5-100马克杯/毫升）刺激ROS 17/2.8成骨细胞样细胞的生长，其效果与E-2和染料木素相同。目前的数据提供了第一个直接的体内证据，红花种子对雌激素缺乏引起的骨质流失有保护作用，但对子宫没有实质性影响。红花种子的有益作用可能是介导的，至少部分是通过多酚类化合物对成骨细胞增殖的刺激作用。
Safflower (Carthamus tinctorius L.) seeds have long been clinically used in Korea to promote bone formation and prevent osteoporosis. However, the beneficial effect has not been scientifically evaluated. Thus, in the present study we investigated whether phytoestrogen rich safflower seeds reduce bone loss in ovariectomized rats. Female Sprague-Dawley rats were subjected to bilateral ovariectomy or sham surgery. One week after the operation, ovariectomized rats were either fed a diet containing defatted safflower seeds or injected with 17beta-estradiol (E-2) for 4 weeks. As expected, ovariectomy resulted in a dramatic reduction in trabecular bone mass of the proximal tibia, increase in deposition of marrow fat, and in uterine atrophy. E-2 treatment almost completely prevented bone loss as well as marrow adiposity, as examined by scanning electron microscopy and histomorphometry. Safflower seeds partially prevented ovariectomy-induced bone loss and slightly reduced marrow adiposity. Safflower seeds, in contrast to E-2, exerted very weak uterotrophic action. In an attempt to elucidate the underlying mechanisms, effect of polyphenolic compounds extracted from safflower seeds on proliferation of osteoblast-like cells was also assessed in vitro. The mixed polyphenolic compounds stimulated growth of ROS 17/2.8 osteoblast-like cells in a dose-dependent manner (5-100 mug/ml), as potently as E-2 and genistein. The present data provide the first direct in vivo evidence that safflower seeds have a protecting effect on bone loss caused by estrogen deficiency, without substantial effect on the uterus. The beneficial effect of safflower seeds may be mediated, at least in part, by the stimulating effect of polyphenolic compounds on proliferation of osteoblasts.