The Shaping of Male Courtship Posture by Lateralized Gustatory Inputs to Male-Specific Interneurons
The Shaping of Male Courtship Posture by Lateralized Gustatory Inputs to Male-Specific Interneurons
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DOI：
10.1016/j.cub.2009.11.038
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发表时间：
2010-01-12
期刊：
影响因子：
9.2
通讯作者：
Yamamoto, Daisuke
中科院分区：
文献类型：
--
作者：
Koganezawa, Masayuki;Haba, Daisuke;Yamamoto, Daisuke
背景：单侧翅膀振动产生“情歌”是黑腹果蝇雄性求爱姿势的一个标志。相比之下，其他一些种类的雄性果蝇在求偶时会同时伸展两只翅膀。因此，不同物种的翅膀运动模式不同，并受到严格的遗传控制，尽管单个物种内的个体之间几乎没有差异。结果：当雄性黑腹潜蝇失去味觉受体基因Gr32a(与信息素受体基因Gr68a有亲缘关系)所介导的味觉输入时，它们在求偶过程中往往不能进行单侧展翅，因为它们在伸展另一只翅膀的同时不能保持一只翅膀处于静止位置。切断一条前腿，而不是其他腿，会增加同时伸翼的发生，这表明该结构中表达Gr32a的细胞参与了求爱姿势的调节。在信息素结合蛋白基因Obp57d失活的雄性中也观察到了类似的同时展翅。表达Gr32a的细胞的轴突投射到食道下神经节，其终末在那里单侧接触MAL，MAL是雄性特有的无果(FU)表达的中间神经元，具有双侧分支。结论：我们的观察有力地表明，味觉信息素的输入确保了符合物种特定行为模式的正确的翅膀振动的偏侧性。
Background: Unilateral wing vibration to generate "love songs" is a hallmark of male courtship posture in Drosophila melanogaster. In contrast, males of some other Drosophila species extend both wings simultaneously during courtship. Thus, the patterns of wing movement vary among species and are under stringent genetic control, although there are few variations among individuals within a single species. These observations prompted the postulation that the proper wing display by courting males of D. melanogaster does not require sensory inputs.Results: Here we show that when males of D. melanogaster are deprived of gustatory inputs mediated by the sensory neurons expressing the taste receptor gene Gr32a, a close relative to the pheromone receptor gene Gr68a, they often fail to perform unilateral wing extension during courtship because they become unable to keep a wing in the resting position while extending another wing. The tarsal amputation of a foreleg, but not other legs, increased the occurrence of simultaneous wing extension, indicating that Gr32a-expressing cells in this structure are involved in the regulation of courting posture. A similar simultaneous wing extension was also observed in males in which the putative pheromone-binding protein gene Obp57d was inactivated. The axons of Gr32a-expressing cells project to the subesophageal ganglion, where their terminals unilaterally contact mAL, which are male-specific fruitless (fru)-expressing interneurons that have bilateral branches.Conclusions: Our observations strongly suggest that gustatory pheromone inputs ensure the correct laterality of wing vibration that conforms to the species-specific behavioral pattern.