Engineering of ribozymes with useful activities in the ancient RNA world
Engineering of ribozymes with useful activities in the ancient RNA world
复制标题
DOI：
10.1111/nyas.12695
复制
发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Mueller, Sabine
中科院分区：
文献类型：
--
作者：
Mueller, Sabine
越来越多的证据表明，在基于DNA和蛋白质的生命出现之前，RNA世界就已经存在了。在这方面，一个长期的研究目标是开发能够催化许多化学反应的功能性RNA。在寻找催化RNA分子复制的RNA酶方面已经进行了大量的努力，包括酶本身。然而，这一问题远未得到解决。除了复制之外，其他有助于提高遗传复杂性和扩展功能空间的功能与RNA世界情景高度相关。我们已经设计了各种发夹核酶后代具有RNA重组，环化和寡聚化的活性。此外，我们已经开发出可以由外部辅因子如黄素单核苷酸（FMN）调节的核酶，并且我们设计了一种RNA催化的RNA氨酰化系统，作为从RNA世界向现代生活过渡的可能途径。
Evidence has been growing that an RNA world existed before DNA-and protein-based life. In this regard, a long-standing research goal has been to develop functional RNAs capable of catalyzing numerous chemical reactions. Much effort has been undertaken in finding RNA enzymes that catalyze the replication of RNA molecules, including the enzymes themselves. This, however, is far from being resolved. Apart from replication, other functionalities that contribute to higher genetic complexity and extended functional space are of high relevance to RNA-world scenarios. We have engineered a variety of hairpin ribozyme descendants possessing activity for RNA recombination, circularization, and oligomerization. Furthermore, we have developed ribozymes that can be regulated by external cofactors such as flavine mononucleotide (FMN), and we have designed a system for RNA-catalyzed RNA aminoacylation as a possible path toward a transition from the RNA world to modern life.