Cytoplasmic estrogen receptors in rat brain: immunocytochemical evidence using three antibodies with distinct epitopes.
Cytoplasmic estrogen receptors in rat brain: immunocytochemical evidence using three antibodies with distinct epitopes.
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DOI：
10.1210/endo.131.3.1380440
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发表时间：
1992-09
期刊：
影响因子：
4.8
通讯作者：
J. Blaustein
中科院分区：
文献类型：
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作者：
J. Blaustein
使用免疫细胞化学技术已报道豚鼠大脑中存在细胞质雌激素受体（ER）。虽然最近在其他物种（例如负鼠、麝鼩和雪貂）中报道了细胞质 ER，但在大鼠大脑中报道了一种完全细胞核的 ER 免疫反应模式。由于所有报道大脑中细胞质 ER 存在的研究都使用了 H 222 单克隆抗体，因此该观察结果可能对该抗体具有特殊性。在本实验中，使用针对 ER 蛋白上不同表位的三种抗体对大鼠脑中的 ER 进行免疫细胞化学染色。对于每种抗体，在下丘脑、视前区、杏仁核和中脑导水管周围灰质中观察到 ER 免疫反应性。在所有情况下，我们观察到细胞核中反应产物的密度最高，但在大多数区域也观察到广泛的细胞质免疫染色。除了证明核周细胞质和大脑细胞质过程中存在神经 ER 免疫反应性之外，这项研究还表明神经细胞质 ER 免疫反应性不仅仅是受体蛋白的一小段；相反，它很可能是整个受体。
The existence of cytoplasmic estrogen receptors (ERs) has been reported in the guinea pig brain using immunocytochemical techniques. While cytoplasmic ERs have been reported recently in other species, such as opossum, musk shrew, and ferrets, an exclusively cell nuclear pattern of ER immunoreactivity has been reported in the rat brain. Because all studies that have reported the existence of cytoplasmic ERs in the brain have used the H 222 monoclonal antibody, the possibility exists that this observation is idiosyncratic to this antibody. In the present experiment three antibodies directed against diverse epitopes on the ER protein were used to immunocytochemically stain ERs in rat brain. With each antibody, ER immunoreactivity was observed in the hypothalamus, preoptic area, amygdala, and midbrain periaqueductal gray. In all cases we observed the highest density of reaction product in cell nuclei, but extensive cytoplasmic immunostaining was observed in most areas as well. In addition to demonstrating the existence of neural ER immunoreactivity in perikaryal cytoplasm and cytoplasmic processes in the brain, this study suggests that the neural cytoplasmic ER immunoreactivity is not just a small fragment of the receptor protein; rather, it is likely to be the entire receptor.