Role of Fgf10 in cell proliferation in white adipose tissue
Role of Fgf10 in cell proliferation in white adipose tissue
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DOI：
10.1016/j.mce.2006.01.010
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发表时间：
2006-04-25
影响因子：
4.1
通讯作者：
Itoh, N
中科院分区：
文献类型：
--
作者：
Konishi, M;Asaki, T;Itoh, N
白色脂肪组织（WAT）的发育包括脂肪形成和细胞增殖。虽然脂肪的形成已被充分研究，但细胞的增殖尚未得到充分研究。因此，我们通过分析脂肪生成和增殖严重受损的Fgf10（-/-）小鼠胚胎WAT来研究其增殖机制。WAT检测细胞增殖必需的d型细胞周期蛋白表达和视网膜母细胞瘤家族蛋白磷酸化。在rgf10(-/-) WAT中，cyclin D2表达和p130磷酸化均受损。在小鼠胚胎成纤维细胞中，F-f10刺激了cyclin D2的表达和p130的磷酸化，这些磷酸化被Ras/MAPK通路的抑制剂抑制。这些结果表明，Fgf10通过Ras/MAPK途径刺激WAT细胞增殖，随后是细胞周期蛋白d2依赖性p130的磷酸化。相反，在Fgf10(-/-) WAT中，pRb的表达受损，而不是磷酸化受损。由于pRb对脂肪形成至关重要，Fgf10可能通过诱导其表达在脂肪形成中发挥作用。2006爱思唯尔爱尔兰有限公司版权所有。
The development of white adipose tissue (WAT) involves adipogenesis and cell proliferation. Although the adipogenesis has been well studied, the cell proliferation has not. Therefore, we examined the mechanism of the proliferation by analyzing Fgf10(-/-) mouse embryonic WAT, in which adipogenesis and proliferation were severely impaired. D-type cyclin expression and retinoblastoma family protein phosphorylation essential for cell proliferation were examined in WAT. Both cyclin D2 expression and p130 phosphorylation were impaired in the rgf10(-/-) WAT. In mouse embryonic fibroblasts, F-f10 stimulated cyclin D2 expression and p130 phosphorylation, which were inhibited by an inhibitor of the Ras/MAPK pathway. These results suggest that Fgf10 stimulates cell proliferation in WAT through the Ras/MAPK pathway followed by the cyclin D2-dependent phosphorylation of p130. In contrast, expression but not phosphorylation of pRb was impaired in the Fgf10(-/-) WAT. As pRb is essential for adipogenesis, Fgf10 might play a role in adipogenesis by inducing its expression. (c) 2006 Elsevier Ireland Ltd. All rights reserved.