Changes in collagenous tissue microstructures and distributions of cathepsin L in body wall of autolytic sea cucumber (Stichopus japonicus)
Changes in collagenous tissue microstructures and distributions of cathepsin L in body wall of autolytic sea cucumber (Stichopus japonicus)
复制标题
自溶海参体壁胶原组织显微结构及组织蛋白酶L分布的变化
DOI：
10.1016/j.foodchem.2016.05.173
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发表时间：
2016-12-01
期刊：
影响因子：
8.8
通讯作者：
Zhu, Bei-Wei
中科院分区：
文献类型：
--
作者：
Liu, Yu-Xin;Zhou, Da-Yong;Zhu, Bei-Wei
通过紫外线（UV）照射诱导海参（Stichopus japonicus）自溶，并利用组织学和组织化学技术研究胶原组织微结构的变化和组织蛋白酶L的分布。新鲜日本参真皮中完整的胶原纤维在紫外线刺激后分解成胶原纤维。在新鲜日本参真皮细胞的液泡表面内鉴定出组织蛋白酶 L。紫外线刺激后，液泡和细胞膜融合在一起，组织蛋白酶L从细胞中释放出来并扩散到组织中。组织蛋白酶L的密度与体壁各层自溶的速度和程度呈正相关。我们的结果表明，溶酶体组织蛋白酶 L 在紫外线刺激下从细胞中释放出来，接触并降解细胞外基质（如胶原纤维），从而参与刺参的自溶。 (C) 2016 Elsevier Ltd. 保留所有权利。
The autolysis of sea cucumber ( Stichopus japonicus) was induced by ultraviolet (UV) irradiation, and the changes of microstructures of collagenous tissues and distributions of cathepsin L were investigated using histological and histochemical techniques. Intact collagen fibers in fresh S. japonicus dermis were disaggregated into collagen fibrils after UV stimuli. Cathepsin L was identified inside the surface of vacuoles in the fresh S. japonicus dermis cells. After the UV stimuli, the membranes of vacuoles and cells were fused together, and cathepsin L was released from cells and diffused into tissues. The density of cathepsin L was positively correlated with the speed and degree of autolysis in different layers of body wall. Our results revealed that lysosomal cathepsin L was released from cells in response to UV stimuli, which contacts and degrades the extracellular substrates such as collagen fibers, and thus participates in the autolysis of S. japonicus. (C) 2016 Elsevier Ltd. All rights reserved.