Distinct frequency dependent effects of whole‐body vibration on non‐fractured bone and fracture healing in mice
Distinct frequency dependent effects of whole‐body vibration on non‐fractured bone and fracture healing in mice
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DOI：
10.1002/jor.22629
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发表时间：
2014-08
影响因子：
2.8
通讯作者：
E. Wehrle;Tim Wehner;A. Heilmann;R. Bindl;L. Claes;F. Jakob;M. Amling;A. Ignatius
中科院分区：
文献类型：
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作者：
E. Wehrle;Tim Wehner;A. Heilmann;R. Bindl;L. Claes;F. Jakob;M. Amling;A. Ignatius
低量级高频振动（LMHFV）在非骨折骨中引起合成代谢效应；然而，在骨折愈合中，报告的结果不一致，最佳振动条件仍未确定。在这里，我们研究了LMHFV对骨折愈合的频率依赖效应。12周大的雌性C57BL/6小鼠接受股骨截骨术，使用外固定架稳定。小鼠接受全身振动（20分钟/天），峰对峰加速度为0.3g，频率为35或45 Hz。10天和21天后，通过弯曲测试、微计算机断层扫描和组织形态测量评估去骨股骨和完整骨（对侧股骨、腰椎）。在未骨折的骨小梁中，35 Hz的振动显著增加了骨的相对数量（+28%）和骨小梁数量（+29%），而皮质骨不受影响。45hz的LMHFV不能引起骨小梁或骨皮质的合成代谢作用。35 Hz的全身振动对骨折愈合没有显著影响，而45 Hz的振动显著降低骨形成（- 64%）和骨痂的弯曲刚度（- 34%）。虽然确切的机制尚不清楚，但我们的研究结果表明，小的振动设置变化可能会显著影响LMHFV对骨重塑和修复中的骨形成的影响，甚至破坏骨折愈合，这意味着在治疗骨折愈合受损患者时要谨慎。©2014骨科研究学会。Wiley期刊公司出版。[J]中华骨科杂志，2014。
Low‐magnitude high‐frequency vibration (LMHFV) provokes anabolic effects in non‐fractured bone; however, in fracture healing, inconsistent results were reported and optimum vibration conditions remain unidentified. Here, we investigated frequency dependent effects of LMHFV on fracture healing. Twelve‐week‐old, female C57BL/6 mice received a femur osteotomy stabilized using an external fixator. The mice received whole‐body vibrations (20 min/day) with 0.3g peak‐to‐peak acceleration and a frequency of either 35 or 45 Hz. After 10 and 21 days, the osteotomized femurs and intact bones (contra‐lateral femurs, lumbar spine) were evaluated using bending‐testing, µ‐computed tomography, and histomorphometry. In non‐fractured trabecular bone, vibration with 35 Hz significantly increased the relative amount of bone (+28%) and the trabecular number (+29%), whereas cortical bone was not influenced. LMHFV with 45 Hz failed to provoke anabolic effects in trabecular or cortical bone. Fracture healing was not significantly influenced by whole‐body vibration with 35 Hz, whereas 45 Hz significantly reduced bone formation (−64%) and flexural rigidity (−34%) of the callus. Although the exact mechanisms remain open, our results suggest that small vibration setting changes could considerably influence LMHFV effects on bone formation in remodeling and repair, and even disrupt fracture healing, implicating caution when treating patients with impaired fracture healing. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1006–1013, 2014.