Characterizing Properties of Biomolecular Condensates Below the Diffraction Limit In Vivo.
Characterizing Properties of Biomolecular Condensates Below the Diffraction Limit In Vivo.
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体内衍射极限以下生物分子凝聚体的特性表征。
DOI：
10.1007/978-1-0716-2663-4_22
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Spille,Jan-Hendrik
中科院分区：
文献类型：
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作者：
Pandey,Ganesh;Budhathoki,Alisha;Spille,Jan-Hendrik
荧光显微镜检测能够直接在其细胞环境中研究内源性生物分子缩合物。通过适当的实验设计，这些测定产生关于冷凝物材料性质的定量信息，并提供关于冷凝物形成的生物物理机制的信息。单分子超分辨率和跟踪实验允许使用常规成像方法无法解决的最小冷凝物和早期冷凝阶段。在这里，我们讨论了使用单分子测定提取定量信息的生物分子凝聚物直接在其细胞环境的考虑。
Fluorescence microscopy assays enable the investigation of endogenous biomolecular condensates directly in their cellular context. With appropriate experimental designs, these assays yield quantitative information on condensate material properties and inform on biophysical mechanisms of condensate formation. Single-molecule super-resolution and tracking experiments grant access to the smallest condensates and early condensation stages not resolved by conventional imaging approaches. Here, we discuss considerations for using single-molecule assays to extract quantitative information about biomolecular condensates directly in their cellular context.