Increased visceral sensitivity to capsaicin after DSS-induced colitis in mice: spinal cord c-Fos expression and behavior
Increased visceral sensitivity to capsaicin after DSS-induced colitis in mice: spinal cord c-Fos expression and behavior
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DOI：
10.1152/ajpgi.00114.2007
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发表时间：
2007-10-01
影响因子：
4.5
通讯作者：
Heijnen, Cobi J.
中科院分区：
文献类型：
--
作者：
Eijkelkamp, Niels;Kavelaars, Annemieke;Heijnen, Cobi J.
DSS诱导的小鼠结肠炎后内脏对辣椒素的敏感性增加：脊髓c-Fos表达和行为美国生理学杂志胃肠和肝脏生理学293：G749-G757，2007年。首次发表于2007年7月26日; doi：10.1152/ajpgi.00114.2007。在急性和慢性炎症期间，内脏疼痛感知发生改变。然而，在炎症后阶段内脏疼痛感知方面存在证据。本研究的目的是调查是否内脏疼痛知觉后，决议葡聚糖硫酸钠（DSS）诱导的结肠炎症改变。通过监测对结肠内辣椒素滴注的行为反应来评估小鼠的内脏感觉功能。两小时后，测定脊髓节段T-12/13-S-1的I/II层和X层中c-Fos阳性神经元的数量作为神经元活化的量度。通过向饮用水中添加1%的DSS来诱导DSS结肠炎。通过确定疾病活动指数评分来评估DSS诱导的结肠炎的病程。动物发生一过性结肠炎，并在第49天恢复。在该时间点，细胞因子水平和结肠长度与对照动物相似。重要的是，DSS诱导的结肠炎消退后，与对照小鼠相比，结肠内辣椒素的行为反应增加。此外，辣椒素诱导的脊髓神经元c-Fos表达显著增加。有趣的是，在结肠炎后，动物也表现出涉及体痛觉过敏，如用腹壁上的von Frey毛测量的。我们的结论是，炎症后内脏痛觉过敏发生DSS诱导的结肠炎的决议后，辣椒素诱导的行为反应和脊髓神经元c-Fos激活是有效的读数确定内脏疼痛的感觉。
Increased visceral sensitivity to capsaicin after DSS-induced colitis in mice: spinal cord c-Fos expression and behavior. Am J Physiol Gastrointest Liver Physiol 293: G749-G757, 2007. First published July 26, 2007; doi:10.1152/ajpgi.00114.2007.During acute and chronic inflammation visceral pain perception is altered. Conflicting data exist, however, on visceral pain perception in the postinflammatory phase. The aim of the present study was to investigate whether visceral pain perception is altered after resolution of dextran sodium sulfate (DSS)-induced inflammation of the colon. Visceral sensory function in mice was assessed by monitoring behavioral responses to intracolonic capsaicin instillation. Two hours later the number of c-Fos-positive neurons in lamina I/II and X of spinal cord segments T-12/13-S-1 was determined as a measure of neuronal activation. DSS colitis was induced by adding 1% of DSS to the drinking water. The course of DSS-induced colitis was assessed by determining the disease activity index score. Animals developed a transient colitis and had recovered at day 49. At this time point, cytokine levels and colon length were similar to control animals. Importantly, after resolution of DSS-induced colitis the behavioral response to intracolonic capsaicin was increased compared with control mice. Moreover, capsaicin-induced spinal cord neuronal c-Fos expression was significantly increased. Interestingly, after colitis animals also exhibited referred somatic hyperalgesia as measured with von Frey hairs on the abdominal wall. We conclude that postinflammatory visceral hyperalgesia occurs after resolution of DSS-induced colitis and that capsaicin-induced behavioral responses and spinal cord neuronal c-Fos activation are effective readouts for determination of visceral pain perception.