EFFECT OF CONOTRUNCAL CONSTRICTION ON AORTIC MITRAL-VALVE CONTINUITY IN THE STAGE-18, STAGE-21 AND STAGE-24 CHICK-EMBRYO
EFFECT OF CONOTRUNCAL CONSTRICTION ON AORTIC MITRAL-VALVE CONTINUITY IN THE STAGE-18, STAGE-21 AND STAGE-24 CHICK-EMBRYO
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DOI：
10.1016/0002-9149(84)90447-8
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发表时间：
1984-01-01
影响因子：
2.8
通讯作者：
ROSENQUIST, GC
中科院分区：
文献类型：
--
作者：
CLARK, EB;HU, N;ROSENQUIST, GC
本文研究了18、21和24期鸡胚圆锥缩窄对主动脉-二尖瓣连续性发育的影响。10-0尼龙缝线系在圆锥动脉干周围，收缩心脏流出道。在236个实验胚胎的3个亚组中，在4或24小时后移除该环或永久保留该环。在心脏形态发生完成后的各阶段采集胚胎心脏，固定于舒张末，并进行显微解剖。用校准的丝状测微器目镜从心底测量二尖瓣和主动脉环之间的距离。对72个正常胚胎和132个对照胚胎的二尖瓣-主动脉分离进行了比较。正常胚胎心脏、正常对照心脏和实验胚胎心脏4、24 h的二尖瓣-主动脉分离度相似。二尖瓣-主动脉间隔由0.34.+-增加。正常心脏0.25 mm降至0.82。+-。0.25毫米，在阶段18，1.11。+-。21期为0.36毫米，+-为0.75毫米。0.33 mm，24期永久循环实验心脏(P<；0.01)。在二尖瓣与主动脉分离度增加的胚胎心脏中，两条大血管均起源于右室，半月瓣位于同一水平，室间隔缺损位于主动脉环下方。圆锥动脉缩窄明显改变了主动脉和二尖瓣的关系。圆锥干缩窄可改变间充质组织向心脏的迁移。
The effect of conotruncal constriction on the development of aortic-mitral valve continuity in stage 18, 21 and 24 chick embryos was studied. A 10-0 nylon suture was tied around the conotruncus constricting the outflow tract of the heart. The loop was removed after 4 or 24 h or left permanently in place in 3 subgroups of the 236 experimental embryos. The embryo hearts were harvested at stages after completion of cardiac morphogenesis, fixed in end-diastole and microdissected. The distance between the mitral and aortic anuli was measured from the base of the heart with a calibrated filar micrometer eyepiece. This measurement was compared with the mitral-aortic separation in 72 normal and 132 control embryos. The mitral-aortic separation was similar among normal, control and 4 and 24 h experimental embryo hearts. The mitral-aortic separation increased from 0.34 .+-. 0.25 mm in normal hearts to 0.82 .+-. 0.25 mm in stage 18, 1.11 .+-. 0.36 mm in stage 21 and 0.75 .+-. 0.33 mm in stage 24 permanent loop experimental hearts (P < 0.01). In embryo hearts with an increased mitral-aortic separation, both great vessels arose from the right ventricle, the semilunar valves were at the same level and the ventricular septal defect was present beneath the aortic anulus. Conotruncal constriction apparently modifies the relation of the aortic and the mitral valve. Conotruncal constriction may alter the migration of mesenchymal tissue into the heart.