Lysobacter caeni sp. nov., isolated from the sludge of a pesticide manufacturing factory.
Lysobacter caeni sp. nov., isolated from the sludge of a pesticide manufacturing factory.
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DOI：
10.1099/ijs.0.000024
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发表时间：
2015-03
影响因子：
2.8
通讯作者：
X. Ye;Cuiwei Chu;C. Shi;Jian-chun Zhu;Qin He;Jian He
中科院分区：
文献类型：
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作者：
X. Ye;Cuiwei Chu;C. Shi;Jian-chun Zhu;Qin He;Jian He
BUT-8（T）是从某除草剂生产废水处理厂的活性污泥中分离得到的一株革兰氏阴性、不运动的杆状好氧菌。比较16 S rRNA基因序列分析表明，菌株BUT-8（T）与溶杆菌属（Lysobacter）聚在一起，与鲁氏溶杆菌（Lysobacter ruishenii）DSM 22393（T）（98.3%）和大田溶杆菌（Lysobacter daejeonensis）KACC 11406（T）（98.7%）的亲缘关系较近。基因组DNA的DNA G+C含量为70.6mol%。主要的呼吸醌是泛醌-8，和主要的极性脂质是二磷脂酰甘油，磷脂酰乙醇胺，磷脂酰甘油和氨基脂质。主要的细胞脂肪酸是异-C15：0、异-C16：0、异-C17：0、异-C11：0、异-C11：0 3OH和总和特征9（包含异-C17：1ω 9 c和/或C16：010-甲基）。菌株BUT-8（T）与其亲缘关系最近的菌株的DNA-DNA相关性低于70%。系统发育学、化学分类学和表型分析结果表明，菌株BUT-8（T）属于溶杆菌属（Lysobacter），并代表一个新种，命名为Lysobacter caeni sp. nov.。模式菌株为BUT-8（T）（= CCTCC AB 2013087（T）= KACC 17141（T））。
Strain BUT-8(T), a Gram-stain-negative, non-motile and rod-shaped aerobic bacterium, was isolated from the activated sludge of a herbicide-manufacturing wastewater treatment facility. Comparative 16S rRNA gene sequence analysis revealed that strain BUT-8(T) clustered with species of the genus Lysobacter and was closely related to Lysobacter ruishenii DSM 22393(T) (98.3 %) and Lysobacter daejeonensis KACC 11406(T) (98.7 %). The DNA G+C content of the genomic DNA was 70.6 mol%. The major respiratory quinone was ubiquinone-8, and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an aminolipid. The major cellular fatty acids were iso-C15 : 0, iso-C16 : 0, iso-C17 : 0, iso-C11 : 0, iso-C11 : 0 3OH and summed feature 9 (comprising iso-C17 : 1ω9c and/or C16 : 010-methyl). The DNA-DNA relatedness between strain BUT-8(T) and its closest phylogenetic neighbours was below 70 %. Phylogenetic, chemotaxonomic and phenotypic results clearly demonstrated that strain BUT-8(T) belongs to the genus Lysobacter and represents a novel species for which the name Lysobacter caeni sp. nov. is proposed. The type strain is BUT-8(T) ( = CCTCC AB 2013087(T) = KACC 17141(T)).